水稻非整倍性基因组表达与表观遗传调控特征研究

非整倍性变异是染色体数目变异主要类型之一。研究表明由于非整倍体基因组处于不平衡状态，因此整个基因组转录和表观遗传调控都会受到影响。本实验室在多年的水稻细胞遗传研究过程中，积累了一系列水稻非整倍性材料，为深入研究非整倍性基因组表达与表观遗传调控特征提供了丰富遗传资源。在前期研究中，我们分析了两种非整倍性基因组的表达特征，并且发现细胞代谢与繁殖相关基因的表达在不同非整倍体中具有共性特征。本研究拟以水稻成套初级三体材料为重点研究对象，以叶片和愈伤组织为研究材料，通过Q-PCR、RT-PCR、ChIP-PCR、MSPCR、RNA-seq、MeDIP-seq、ChIP-seq等研究技术明确水稻基因表达特征和表观遗传调控特征。并结合基因功能分析及其对表型、分裂行为的影响，挖掘非整倍性基因组在细胞代谢和繁殖相关基因表达、表观遗传调控特征，构建相关基因表达与表观修饰调控网络。

Aneuploidy involves changes in chromosomal copy number compared with normal euploid genotypes. Aneuploidy causes severe developmental defects and is a condition frequently found in tumor cells. Aneuploidy genome is in the state of unbalance,which affects the global genome transcriptional and epigenetic regulation. Studies of gene expression in aneuploids in a variety of species have claimed many different types of responses. Studies of individual genes suggest that there are both structural gene dosage effects and compensation in aneuploids, and that subtle trans-acting effects across the genome are quite prevalent. Moreover，researches of epigenomic in aneuploidy in less species have widespread alterations, such as DNA methylation and histone modification..Although the phenotypic manifestations of aneuploidy are usually apparent, information about the underlying alterations instructure, expression, and epigenetic modification of aneuploidy genome is still sparse. Plants generally tolerate aneuploidy better than animals. Through colchicine treatment and breeding strategies, we have obtained a set of trisomy in rice. This possibility, combined with the genomics and epigenetics tools available for rice, provides a powerful means to assess systematically the epigenomic and transcriptomic signatures of aneuploidy. In preliminary studies, we analyzed two kinds of aneuploidy genome expression characteristics. The results showed that the expression of genes involved in cell metabolism and reproduction has common characteristics in different aneuploidy genome. .In this project, We are going to profile gene expression, genome-wide specific DNA methylation and histone modificationin a set of trisomic byusing the following approaches: Q-PCR, RT-PCR, ChIP-PCR, MSPCR, RNA-seq, MeDIP-seq and ChIP-seq. The results will reveal characteristics of gene expression and epigenetic regulation in rice aneuploidy genome. We will focus on the key to exploit the characteristics of genes involved in cell metabolism and reproduction. Furthemore, combined with gene functional analysis and its effect on the phenotype, cell division behavior, the results will provide the construction of regulation network associated with gene expression and epigenetic modifications.