本研究通过干扰RNA技术，沉默人肝癌细胞系HepG2中的OCT4-pg1或OCT4基因，探索两者之间的相互影响及相互调控；通过pCMV/OCT4-pg1 3'UTR病毒载体的构建，研究其对肝癌细胞增殖的调控作用及其作用通路；通过筛选与OCT4-pg1和OCT4基因有共同作用的microRNAs， 研究两种mRNA竞争性地与靶标miR-145结合，探索OCT4假基因调控OCT基因的机制；检测OCT4-pg1和OCT4基因在裸鼠肝癌组织中的表达情况，研究敲除或高表达OCT4-pg1对裸鼠肝癌生长的影响；通过以上研究，明确OCT4的假基因转录后的非编码RNA是否具有功能、能否影响OCT4基因的表达，来阐明假基因对肿瘤生物学的影响和调控，为临床肿瘤诊断、治疗提供全新的思路和途径。

Our study aims to explore mutual influence and regulation between OCT4-pg1 and OCT4 through knockdown OCT4-pg1 and OCT4 genes in human liver cancer cell line HepG2 by small interfering RNAs(siRNAs). Constructing viral vector pCMV/OCT4-pg1 3'UTR to clarify the regulation of OCT4-pg1 to the proliferation of liver cancer cell and associated pathways. Selecting miRNAs that can co-bind with OCT4 and OCT4-pg1, to illuminate the competitive combination of miR-145 to both OCT4 mRNA and OCT4-pg1 mRNA, to further discuss the mechanism how OCT4 pseudogene regulates OCT gene. To examine the expression of OCT4 and OCT4-pg1 in liver cancer tissue in nude mice. Further more, to find out knock-down or over expression of OCT4-pg1 would exert influence on tumor growth in nude mice. Upon this study, clarifying whether non-coding RNA of OCT4 pseudogene has function, whether could impact the expression of OCT4 gene, to illucidate the regulatory function of pseudogene to tumor biology, which could further provide a brand new perspective for clinical diagnosis and treatment.