针对脓毒症复杂体系的特点，申请者在前期研究中采用多种组学技术发现了MMRN1、PTX3、IL-1R2等一批新的脓毒症生物标志物，揭示了其上游的部分转录因子和microRNA分子，以及C/EBP、PPAR-γ、内皮抑制素等可能的脓毒症干预靶点。基于上述前期工作基础，本项目以生物标志物为切入点，采用组学技术与生物学技术相结合，试图发现一批可用于脓毒症诊断、预后判断、病原体鉴别的新的生物标志物群；以这些生物标志物群为基础，揭示其上游的关键转录因子群和关键microRNA分子群，从系统水平揭示脓毒症的发病机制；以上述关键生物标志物群、转录因子群、microRNA分子群为基础，进行相互作用组学分析，揭示脓毒症的某些关键靶点，探讨能干预多个靶点的整合性脓毒症干预新方案。

In the previous studies, in view of the characteristics of sepsis as a complex system, the applicant by using omics approaches found several new sepsis biomarkers such as MMRN1, PTX3 and IL-1R2, revealed some of their upstream transcription factors and microRNAs, and some possible intervention targets such as C/EBP, PPAR-γ, and endostatin. Based on above previous work, this project is attempted to find a batch of biomarkers which can be used for diagnosis, prognostic prediction and pathogen identification of sepsis by choosing biomarkers as a pointcut and employing combination of omics technologies and biologic technologies. Based on the information of above biomarkers, their upstream key transcription factors and key microRNAs will be further explored, so as to reveal the pathogenesis of sepsis on a systematical level. Based on above key biomarkers, key transcription factors and key microRNAs, interactomics analysis will be carried out so as to reveal some key intervention targets of sepsis, and explore some new integrated intervention schemes which aim at multiple targets of sepsis.