布鲁氏菌病是一种严重危害公共卫生安全的人兽共患传染病。苍白杆菌是一种致病力很弱的条件性致病菌，与布鲁氏菌的亲缘关系很近，但其致病性却差异很大。 比较基因组学发现，在苍白杆菌中缺少布鲁氏菌中的二元调控系统TceSR和TcfSR，且其功能及其毒力尚不清楚。本研究以二元调控系统TceSR和TcfSR为切入点，系统分析二元调控系统TceSR和TcfSR缺失株毒力表型，筛选验证受其调控的布鲁氏菌致病基因与蛋白。通过RNA-Seq技术筛选受TceSR和TcfSR调控的sRNA和mRNA分子；生物信息学预测调控sRNA的靶基因并进行验证，深入研究TceSR和TcfSR的调控通路，全面阐述二元调控系统TceSR和TcfSR调控布鲁氏菌致病性基因的分子机制，为深入阐明布鲁氏菌的致病机制和研发有效的治疗药物的靶点和新型疫苗提供科学依据。

Brucellosis is a serious hazard to public health and safety of zoonotic infectious disease. Ochrobactrum anthropi (O. anthropi) is a weak pathogenicity conditioned pathogen, which phylogenetic relationship is very close to Brucella. But the pathogenicity between them is very different. Comparative genomics found that O. anthropi lacks orthologs of the Brucella TCSs TceSR and TcfSR, which roles and virulence are still unclear. In this study, the two-component regulatory system (TCS) TceSR and TcfSR were studied, the virulence phenontypes of TCS TceSR and TcfSR mutants were analyze, virulence genes and proteins of Brucella regulated by TCS TceSR and TcfSR were verificated and identified. We found that O. anthropi lacks orthologs of the Brucella TCSs TceSR and TcfSR through comparative genomics analysis. This project will expound the molecular regulatory mechanism of pathogenic gene of Brucella regulated by TCS TceSR and TcfSR. The results of this project will provide the scientific foundation for Brucella pathogenic mechanism clarification, researching and developing the effective therapeutic drug targets and novel vaccine. The sRNA and mRNA regulated by TCS TceSR and TcfSR were verificated through RNA-Seq. The target genes of regulated sRNA were predicted and identified through bioinformatics and the regulatory pathway of TCS TceSR and TcfSR was intensive studied.