植物作为重要的先导化合物来源之一，从中发现具有抗病毒活性天然产物是申请者所在课题组近年来重要的研究方向之一。前期研究显示，从外来入侵植物三裂蟛蜞菊（ Wedelia trilobata (L.) Hitchc.）中发现的桉烷型倍半萜内酯类化合物具有很强的抗烟草叶病毒（TMV）的活性，是本研究组发现的强抗病毒活性成分之一。深入的研究提示该类化合物抗TMV病毒的活性有可能与其诱导植物自身产生系统获得性抗性有关。本课题拟对该类化合物抗TMV活性进行深入研究，探讨该类化合物抗TMV的构效关系，并在此基础上，以活性显著的化合物作为小分子探针，探讨其作用机制和作用靶蛋白，以此帮助我们深入系统地认识桉烷型倍半萜类化合物抗TMV的途径，从而发现具有自主知识产权的抗TMV先导化合物，为我们今后从植物中寻找具有抗病毒天然先导化合物提供理论基础。

Plants is one of the important sources of lead compounds , searching for antiviral activity of natural products is one of the important research direction of the applicant's group in recent years, Pilot study on the invasive plants (Wedelia trilobata (l.) Hitchc.) found that eudesmanolides has the very strong resistance activity to TMV, is one of strong antiviral active ingredients. In-depth research show that eudesmanolides against TMV activity may be related to its induced plant itself to engender system acquired resistance. This project focus investigation of structure-activity relationship, targeting protein and machanism of anti-TMV for pancratistatin-type alkaloids, in order to find the lead compounds and clarify their effecting mechanism.