本项目以人正常血管、内膜增生的肾血管组织和分化型、去分化型主动脉平滑肌细胞(HASMC)为研究对象，应用MiRNA芯片、ChIP sequencing, RNA干扰、KLF5修饰位点突变、Oligo pull down、EMSA和报告基因分析等技术研究研究KLF5苏素化调节VSMC特异基因表达的分子机制，探明KLF5苏素化修饰与组蛋白乙酰化/去乙酰化以及甲基化/去甲基化等表观遗传学变化的关系，明确MiRNA调节KLF5苏素化及VSMC表型重塑的组合作用模式，从表观遗传学角度探讨VSMC表型转化的分子机制，为防治血管重塑性疾病提供新的治疗依据和药物靶点。

In this study, we used human normal or vascular hyperplasia tissues, differentiation or dedifferentiation human aortic smooth muscle cells as target, to investigate the role of KLF5 SUMOylation and mechanism of KLF5 actions in regulating VSMC specific genes expression as well as histone acetylation using MiRNA arrays, ChIP sequencing, RNA interference, KLF5 modification sites mutation, Oligo pull down, EMSA and reporter gene assay, etc. The aim is to explore the relationship between KLF5 SUMOylation and acetylation/deacetylation, methylation/ demethylation of histone H3 and H4, identify the model and mechanism of MiRNA regulated the KLF5 SUMOylation and VSMC remodeling. These researches will further find out the molecular mechanism manipulating the VSMC phenotype from the epigenetic aspect and provide a new therapeutic basis and target of vascular remodeling diseases.