毛细血管渗漏综合征(CLS)是重症急性胰腺(SAP)全身炎症反应综合症向多器官功能衰竭的中间阶段，治疗CLS对改善SAP的预后有重要的临床意义。我们前期发现了SAP相关性miRNA（miRNA551b-5p），通过预实验，观察到它会引起内皮细胞的通透性变化。其中跨细胞途径及细胞旁途径是CLS的主要机制，跨细胞途径与水通道蛋白有关，细胞旁途径与细胞连接有关，我们还发现急性胰腺炎相关性miRNA通过EGFR与内皮细胞通透性相关。因此，我们拟扩大样本量，进一步证实miRNA551b-5p与CLS的关系；通过上调及下调miRNA551b-5p，研究其在细胞水平和生物个体水平中对CLS影响的分子机制，并结合测序、荧光素酶等技术，对相应基因功能及作用机理筛查和验证，特别是EGFR基因在CLS的的作用，以了解这些基因的功能在CLS中的机制。为人们认识在SAP中发生CLS的机制及治疗提供新思路。

Capillary leakage syndrome is the intermediate stage from systemic inflammatory response syndrome to multiple organ failure in the patients of severe acute pancreatitis . The treatment of capillary leakage syndrome has very important to the patients of severe acute pancreatitis. Our previous studies have found that the lever of miRNA551b-5p in the patients of severe acute pancreatitis is higher than the patients of mild acute pancreatitis.The transcellular pathway and the paracellular pathway are the main mechanisms of capillary leakage syndrome. The transcellular pathway is mainly related to aquaporin protins.Paracellular pathway is mainly mediated by cellular junctions. We had found that miRNA associated with acute pancreatitis was related with tight and adhesive junctions and aquaporin protins by the genes of EGFR yet.Therefore, we intend to expand the number of samples to further confirm the relationship between miRNA551b-5p and capillary leakage syndrome. Combineing with sequencing we research the molecular mechanism of capillary leakage syndrome at the cellular and biological individual levels by up-regulating and down-regulating miRNA551b-5p. The function and mechanism of EGFR gene were screened and verified by two color fluorescence complementary verification techniques, especially the role of EGFR gene in capillary leakage syndrome. The study will help us to understanding the function of these genes related miRNA551b-5p in the mechanism of capillary leakage syndrome. The results of the study will provide a new way for people to further understand the pathogenesis and treatment of capillary leakage syndrome in the patients of severe acute pancreatitis .