脂肪干细胞促进血管内皮祖细胞增殖和分化治疗糖尿病性勃起功能障碍及其机制的研究

糖尿病性勃起功能障碍(DED)发病率高、疗效差，其核心发病机制是阴茎海绵体血管内皮功能障碍。单一的内皮祖细胞(EPCs)移植疗效不佳，我们研究发现EPCs虽可分化为血管内皮细胞，但因移植后其增殖、分化能力下降，限制了其疗效和应用。我们进一步研究发现脂肪干细胞(ADSCs)可促进EPCs增殖和分化，但其作用机制尚未阐明。因此，我们提出通过ADSCs促进EPCs增殖和分化来修复受损的血管内皮功能，从而治疗DED的科学设想。本项目拟通过ADSCs和EPCs体外共培养实验和在DED大鼠模型上的联合移植实验，采用免疫组化、Western blot、RT-PCR等方法，旨在获得ADSCs促进EPCs增殖和分化的可靠证据，评价ADSCs和EPCs联合移植治疗DED的有效性和安全性，本项目将阐明ADSCs促进EPCs增殖和分化的作用机制，为确立EPCs和ADSCs联合移植作为DED治疗新方法提供科学依据。

The core of the pathogenesis of diabetic erectile dysfunction (DED) is the penile endothelial dysfunction, and the existing treatment is not effective enough. Endothelial progenitor cells (EPCs) can differentiate into vascular endothelial cells and improve erectile function, but after they transplant their decreased proliferation and differentiation limit their efficacy and application. We found that adipose stem cells (ADSCs) can promote the proliferation and differentiation of EPCs, but the mechanism is not fully elucidated. Therefore, we first proposed that ADSCs can promote the proliferation and differentiation of EPCs and treat DED by repairing damaged penile endothelial function. We intend to carry out the co-culture experiments of ADSCs and EPCs, and the transplantation experiments in the DED rat model, using immunohistochemistry, PCR and other methods, in order to obtain reliable evidence of ADSCs promoting the proliferation and differentiation of EPCs in vitro and in vivo, reveal its mechanism from the cellular and molecular levels, and evaluate the efficacy and safety of ADSCs and EPCs transplantation to treat DED. The project will clarify the mechanism of ADSC promoting the proliferation and differentiation of EPCs, and provide adequate scientific basis for transplantation therapy of DED.