改良家蚕丝腺合成的蛋白质纤维成分和有效利用丝腺的蛋白高效分泌能力生产外源蛋白，是养蚕业和昆虫生物工程领域世界瞩目的重大课题。本项目选择在中部丝腺特异表达的丝胶蛋白Sericin A (SER3) 编码基因Ser3，后部丝腺 (PSG) 特异表达的丝素蛋白重链 (Fib-H) 的人工改造编码序列 (Hpl)，通过转基因技术分别将Ser3和Hpl在家蚕PSG高效表达，进一步建立具有典型转基因家蚕代表性的丝腺正常发育模型SER(Ser3/Ser3)和PSG异常发育模型TBH(Hpl/Hpl)。通过比较研究动物模型的丝腺组织发育，特别是PSG的蛋白质合成、分泌和相关代谢调控变化，探讨转基因家蚕SER和TBH的PSG蛋白质合成和分泌效率的差异机制，为开发新型蚕丝蛋白和丝腺高效表达外源蛋白的家蚕积累基础。

Improving protein fiber composition synthesized by silk gland and taking use of silk gland to produce exogenous protein efficiently are the major issue in sericulture and insect bio-engineering field. Using transgenic technology, we have made the posterior silk gland (PSG) efficiently express middle silk gland specific protein Sericin A (SER3) coded by Ser3 gene and the artificially modified coding sequence (Hpl) of silk fibroin heavy chain (Fib-H) which is specially expressed in PSG, further established two genetically modified silkworm models with typical representation, normal silk gland development model SER(Ser3/Ser3) and PSG abnormal development model TBH(Hpl/Hpl). Through comparing silk gland tissue’s development of two models, especially changes in protein synthesis, secretion and relevant metabolic regulation in PSG to elucidate their differential regulatory mechanism in silk gland cells. Eventually we aim to clarify the regulatory mechanism which makes silk gland efficiently express exogenous protein, and lay a foundation to exploite new type of silk protein in silkworm.