生物钟信号途径与生物代谢和功能调控密切相关。温度和光照等生物钟授时信号，如何在分子水平输出并与家蚕内分泌代谢途径发生关联，是一个几乎空白、同时影响养蚕生产和害虫防治应用的关键科学问题。本项目选择家蚕滞育这一研究生物钟信号途径对代谢调控的优势性状，通过建立持续性干涉核心钟基因Per, Tim和Cry的遗传突变卵巢细胞系，研究不依赖于内分泌调控的生物钟系统与滞育代谢的联系。进一步建立敲除Per或Cry的遗传突变体家蚕，阻断生物钟输入通路和核心振荡器作用，比较研究突变体蚕蛹组织及所产卵的糖代谢这一滞育特征性代谢变化，挖掘家蚕生物钟系统的输出通路成员和糖代谢调控因子，探讨生物钟系统与内分泌系统对滞育代谢的协同调控机制，为深入研究温度授时因子的作用机制建立技术平台。

The circadian signal pathway is closely related to the metabolic regulation. How the zeitgeber like temperature and light can relate to endocrine metabolism is a key scientific issue. It is almost blank and plays an important role in sericulture production and pest control. In this project we choose diapause, which is a prepotent character to reveal the relationship between circadian clock and metabolic regulation. Then we establish genetic mutations of BmN cell lines, which silence the core circadian genes like Per, Tim and Cry consistently. After that, we will study the relationship between circadian clock genes and diapause metabolism which is independent of endocrine system. Furthermore, we will establish the genetic mutation strains of Bombyx mori such as Per and Cry knockout mutants to research the carbohydrate metabolism changes, which is a characteristic diapause metabolic change. Also, we can dig more output pathway members of circadian clock, and then establish the relationship between endocrine metabolism and diapause metabolic. This work will establish a technology platform for further investigation about temperature zeitgeber.