缘毛类纤毛虫是最早被发现的原生动物类群，具有三百多年研究历史，但仍存在一系列系统发育学问题亟待解决。我们以游走目代表种、鱼病重要病原显著车轮虫，固着目代表种钟形钟虫、螅状独缩虫、树状聚缩虫、褶累枝虫和伞状聚钟虫为研究对象，在测定这六种缘毛类纤毛虫大核基因组的基础上，通过鉴定更多的直系同源基因（尤其是结合生物信息和转录组/蛋白组实验结果鉴定编码帚胚和附着盘等反口区结构的基因），进而基于这些基因重建系统发育树，利用系统发育基因组学研究方法探讨缘毛类与寡膜纲其它亚纲类群间、缘毛亚纲内游走目与固着目间、及各有柄固着类间的系统发育关系，不仅将首次获得缘毛类基因组数据、大大丰富纤毛虫基因组资源，在基因组水平解决缘毛类的系统发育学问题；而且将建立开展纤毛虫系统发育基因组学研究所需的策略和方法，开启该研究领域的大门；同时也将探讨游走缘毛类适应寄生生活的分子机制，为寻找防治车轮虫病的药物靶标提供重要线索。

Peritrichous ciliates have been known for more than 300 years as a distinct group, but there are a series of puzzling and unsolved phylogenetic problems of peritrichs. Selecting one representative species of mobline (a pathogens of fish disease, Trichodina nobilis) and five representative species of sessilines with the stalk ( Vorticella campanula, Carchesium polypinum, Zoothamnium arbuscula, Epistylis plicatilis and Campanella umbellaria) , we sequence their macronucleus genomes, and identify more orthologous genes (especially identify the genes encoding the components of adhesive disk, scopular, stalk and spasmoneme etc. combing the bioinformatic predictions and experimental data from transcriptome and proteome of adhesive disk and stalk), and construct the phylogentic trees using these gens, finally discuss the phylogenetic relationships among peritrichs and other subclasses of Class Oligohymenophorea, between moblines and sessilines, and among sessilines with stalk. The completion of this project will firstly gain the genomic data of peritrichs enriching the genomic resources of ciliates, and resolve the phylogenetic problems of peritrichs at the genome level; and open the door of phylogenomics of ciliates by setting up the strategy and technology for this field; also discuss the molecular mechanisms of Trichodina adapting to parasitic life and find some clues of drug targets for Trichodina disease.