胆碱磷酸功能化自组装薄膜的制备及其抗污性能研究

本项目中，为解决蛋白质在生物材料表面的不良吸附问题，我们设计并合成了三种新型烷基胆碱磷酸分子，并通过囊泡自融合组装技术，构建具有抗污潜能的胆碱磷酸自组装薄膜。系统研究溶液pH值、离子强度、温度等因素对其自组装过程的影响。并将耗散型石英晶体微天平技术与原子力显微镜、拉曼光谱等技术相结合，考察不同等电点蛋白质与胆碱磷酸薄膜间的相互作用过程，从分子水平上揭示并理解胆碱磷酸分子阻抗蛋白吸附的作用机理；进一步利用耗散型石英晶体微天平研究正常细胞和肿瘤细胞在薄膜表面的早期粘附过程，绘制细胞吸附动力学曲线，探究胆碱磷酸分子促进细胞粘附的作用机制。此项目将为新型两性离子抗污材料的设计和开发提供实验依据和理论基础。

In this proposal, we synthesize three Alkyl choline phosphates (CP) to involve the problems of protein adhesion on biomaterial surface. The self-assembled choline phosphate film has been prepared by vesicle fusion method. We will study the effects of pH, ionic strength, solution temperature and et al. on the self-assemble process. Meanwhile, we will investigate the interaction process between proteins and the CP-film by combing quartz crystal microbalance with dissipation (QCM-D) with atomic force microscope (AFM) and Raman spectroscopy and so on, and understand the mechanism of the CP-film resisted proteins under molecule level. And then, we will monitor the early cellular adhesion behavior of CP-film to cells in real time. Therefore, we can solve the kinetic process of the adhesive behaviors. Based on the results of this project, we can get more data to better develop CP-film for using protein resistant materials.