氯胺酮作为新型毒品而被广泛滥用，特别受到青年人的青睐,氯胺酮成瘾危害严重，寻找有效的戒毒方法具有重要意义。针灸已被广泛用于临床治疗药物成瘾且取得一定的疗效，但其作用机制尚不明确。大多数成瘾性药物主要是通过作用于中脑边缘系统介导成瘾药物奖赏效应的核心部位--中脑腹侧被盖区和伏隔核，引起多巴胺通路发生适应性改变，从而导致成瘾。本课题将在前期相关药物成瘾研究的基础上，建立恒河猴氯胺酮成瘾模型，并给予电针治疗；通过脑fMRI扫描观察和分析恒河猴氯胺酮成瘾关键性奖赏脑区的结构及功能活动变化规律，以及电针治疗对这些脑区的影响；并以DNA甲基化修饰介导调节基因表达作为研究切入点，运用免疫组化、Western blot、RT-PCR、焦磷酸测序等分子生物学的方法和技术，从整体-组织-细胞分子水平进行研究，探讨电针治疗氯胺酮成瘾疗效的作用机制，将为临床推广应用电针治疗药物成瘾提供科学依据。

Ketamine is widely abused as a new drug, which is especially popular with young people. Since Ketamine addiction poses health hazard, it is very important to find the effective method of detoxification.Acupuncture has been widely used in the clinical treatment of drug addiction and achieved a certain effect, but its mechanism is still unclear.Most addictive drugs mainly acted upon the mesolimbic system mediated drug reward -- the core parts of the ventral tegmental area and the nucleus accumbens, which results in adaptive changes in the dopamine pathway and thereby leads to addiction.On the basis of our previous studies on drug addiction, this study was designed to establish rhesus monkey models of ketamine addiction by conditioning stimulation of rings and then giving them Electroacupuncture (EA) treatment. The changes of the structure and function of the critical brain regions of ketamine addiction in monkeys were observed and analyzed by brain fMRI scanning, and the effects of EA treatment on these brain regions were observed as well.Moreover, the DNA methylation mediated regulation of gene expression was taken as the starting point,and with the help of the methods and techniques of immunohistochemistry, Western blot, RT-PCR, pyrosequencing and other molecular biology research, this study explored the mechanism of EA treatment of ketamine addiction from the overall organization of cellular and molecular level,which will provide scientific basis for the clinical application of EA in the treatment of drug addiction.