自然流产（SA）指妊娠自然终止，多发生在妊娠早期。蜕膜细胞（DSC）功能失衡是SA发生的重要环节。前期研究证实补肾安胎复方对蜕膜细胞功能的保护作用，采用转录组高通量测序（mRNA-Seq）筛选SA蜕膜组织中过表达的PSG基因家族，基因芯片技术筛选同源低表达的miRNAs，经生物信息学分析miRNAs介导PSG基因家族mRNA表达，提出：减味寿胎丸可能通过miRNAs介导SA蜕膜细胞的PSG基因家族mRNA表达而维持妊娠。故根据肾主生殖，以离体蜕膜细胞为载体系统研究：大样本验证SA蜕膜组织中PSG和miRNAs的表达，并与SA临床指标关联；下调正常早孕蜕膜细胞miRNAs，上调SA蜕膜细胞miRNAs并用siRNA沉默PSG基因家族，检测PSG基因家族mRNA和蛋白表达及蜕膜细胞表型变化；减味寿胎丸全提取物干预SA蜕膜细胞，阐释补肾安胎法通过介导miRNAs调控PSG基因家族表达的作用机制。

Spontaneous abortion（SA）is the natural termination of pregnancy which occurs at first trimester.Unblanced function of the Decidual stromal cells (DSC) plays an important role during SA .We’ve already comfirm that Minus ShouTai pills which are used to tonifying kidney and preventing miscarriage can protect the function of the DSC. High-throughput sequencing of mRNAs (mRNA-Seq) was used in preliminary study to detect the mRNAs of PSG (Pregnancy specific beta-1-glycoprotein) family which was over-expressed in decidua from women suffering from SA. Microarray analysis was used to detect the miRNAs under-expressed in decidua with the same origin as former. .Therefore , we put forward the hypothesis that the main mechanism of Minus ShouTai pills which can protect the function of decidual cells in SA may mediated by miRNAs that regulate the expression of PSG family .Therefore , based on the theory that kindey is primarily responsible for reproduction, we intend to use DSC cultured in vitro as the target of our systematic study. Largely detect mRNA of PSG gene family and miRNAs and link them with the clinical index .Down regulate and up regulate the miRNAs in DSC with siRNA transfection of DSC to silence the PSG gene family .Then, detect the expression of PSG gene family and the phenotype of DSC. Intervene the DSC .with the extract of Minus ShouTai pills,thus we can inllustrate the mechanism of therapies used for tonifying kidney and preventing miscarriage that was mediated by miRNAs which regulate the expression of PSG family.