蜱作为常见外寄生虫和多种疾病的传播媒介，在兽医和公共卫生上具有重要意义。控制蜱的繁殖是防治蜱的中心环节，因此了解雌蜱的生殖调控机制十分必要。蜱的生殖与营养吸收关系密切，吸血-消化-产卵被称为一个生殖营养周期。为了阐明镰形扇头蜱的丝氨酸蛋白酶抑制分子（Serpin）RHS-2严重影响雌蜱饱血和产卵的现象，本研究开展该分子在雌蜱血液消化和生殖产卵上的作用机制研究，探索其在蜱肠道的动态变化规律和调节消化酶的作用，并通过蜱粪便的蛋白组学比较，研究其对血液蛋白消化的影响范围和程度；根据卵黄蛋白与卵黄原蛋白的相同的免疫原性特点，应用免疫学技术分析RHS-2对卵黄原蛋白合成位点、转运、分布和血淋巴中含量等卵黄发生关键环节的调节机制，结合对卵巢和胚胎的形态学观测，研究其对生殖产卵的影响机制。研究结果对阐明Serpin分子在生殖营养周期中的作用机制，发现新的蜱控制策略具有重要意义。

As the common ectoparasite and vector of many diseases, the tick has the vital significance in veterinarian and in the public health. Reproduction interdiction is the key step for tick control; therefore, understanding the female tick's reproduction regulation mechanism is very important. In order to explore the phenomenon that serine proteinase inhibitor (Serpin) RHS-2 affects seriously the female tick gonotrophic cycle, this research will study mechanism of the tick Serpin molecular RHS-2 on the female tick blood digestion and reproduction using the RNA interference, the enzyme interaction, proteomic and immunology analysis methods. It will be explored that RHS-2 dynamic changes and interaction with digestive enzymes in midgut, and the influence profiles on the blood protein digestion by feces proteomic analysis . Based on same immunogenic characteristic between the vitellogenin and vitellin protein, we will analyze vitellogenin’s the synthesis position, transportation, distribution and amounts in the tick hemolymph by immunologic methods. Together with the ovary and embryo morphology observation, we will conclude the mechanism of RHS-2 on the reproduction. The findings has the vital significance in expounding the Serpin member role in digestion and reproduction around the female tick gonotrophic cycle, and it will be useful to reveal tick's reproduction regulation rule, to provides the new way for tick's preventing and controlling strategy.