本项目面向国内养殖业重大疫情和临床诊断对高灵敏低成本的数字PCR（dPCR）核酸检测的巨大需求，针对当前商品化dPCR仪购置及使用成本高昂，集成度低带来的操作复杂等问题，开展低成本全集成的液滴数字PCR（ddPCR）方案研究。发展创新的基于毛细管的快速液滴生成方法和技术，在自主知识产权的序控液滴阵列系统平台上集成样品前处理、液滴生成、PCR扩增、液滴荧光检测和数据分析全过程。在降低成本的同时实现了整个分析流程的集成化和自动化，最终将系统应用于非洲猪瘟病毒和结核杆菌等微生物病原体的高灵敏定量分析。研究争取在毛细管快速液滴生成、多体积液滴陈列生成、二维液滴阵列的荧光成像检测、集成预封装试剂的ddPCR试剂盒、以及临床微生物核酸ddPCR检测等方面上获得突破性的进展，为dPCR技术的发展和普及，也为养殖业和临床诊断病原微生物的高灵敏检测做出应有的贡献。

In view of the high price and complicated operation of the current commercial dPCR instrument, this project aims to develop a low-cost fully integrated ddPCR analysis system to meet the huge demand for aquaculture and clinical diagnostic. By developing capillary-based rapid droplet generation techniques, the whole process of dPCR, including sample pretreatment, droplet generation, PCR amplification, droplet fluorescence detection and data analysis is integrated on the platform of the sequential operation of droplet array system. The system will be applied to quantitative analysis of microbial pathogens such as African swine fever virus and Mycobacterium tuberculosis. The research strives to achieve breakthroughs in capillary rapid droplet generation, multi-volume droplet array generation, droplet arrays fluorescence imaging detection, reagent-preloaded ddPCR kits, and clinical microbial nucleic acid ddPCR detection. This will contribute to the promotion and popularization of dPCR technology, and also contribute to the highly sensitive detection of pathogenic microorganisms in animal husbandry and clinical diagnosis.