菌株高浓度糖基转运能力差和代谢产物的胞内累积抑制产物转化等难题一直困扰着发酵工业，是限制节能环保大宗化学品浓醪发酵工业发展的“瓶颈”问题，几乎处于研究空白。本研究以自主产权高糖高产醇工业酵母C.glycerinogenes为研究对象，基于前期研究基础及所建立独有分子操作平台，采用生物信息学及组学、定向筛选、功能互补等技术发掘高浓度糖醇转运蛋白新成员，并通过同位素液体闪烁计数技术、荧光蛋白标记、DNA pull-down、功能区突变、表面等离子共振、COIP等手段，从糖醇转运蛋白的高浓度转运动力学、mRNA动态应答和后修饰调节等方面进行研究，揭示尚未知的“高浓度下物质的快速转运及其机制”，为浓醪发酵难题的解决及菌株改造提供新理论、基因资源及新途径。

High gravity fermentation is an energy-saving and environmentally friendly strategy to produce chemicals. However, the poor transportability of the substrate in the high glucose matrix and the inhibition of product synthesis by the accumulation of intracellular metabolites are a huge challenge and bottleneck for the high gravity fermentation industry. Moreover, current research does not cover this area. Candida glycerinogenes is a multi-stress tolerance industrial strain with a proprietary property and high alcohol titer at high sugar concentration. Based on our previous study and established genetic manipulation platform, the efficient sugar and alcohol transporters of C. glycerinogenes and their potential regulation mechanism will be studied. Bioinformatics and omics, library screening, and gene function complementary will be applied to discover novel genes responsible for the transmembrane transportation of sugar and alcohol under high concentrations. The high-concentration transport kinetics, mRNA dynamic response and post-translational modification regulation of sugar alcohol transporters will be analyzed by muti-strategies, such as isotopic tracer, fluorescent protein labeling, DNA pull-down, site-specific mutagenesis, surface plasmon resonance, and Co-Immunoprecipitation. The findings achieved here will demonstrate the unknown mechanism of material transcription and protein regulation of transporters under high concentration of sugar and alcohol and provide new theories, genetic resources and new ways for the solution of high gravity fermentation problems and strain modification.