发酵工业菌株对底物产物耐受（抗逆）性差是共性问题，是急需解决的严重影响发酵工业发展的“瓶颈”之一，项目以自主产权耐高渗工业酵母的抗逆CgHOG应答调控途径为研究对象，在本团队四十五年对该酵母的研究和已完成的全基因组测序基础上，利用生物信息学、分子生物学等手段进一步确认CgHOG应答关键基因及转录因子；通过转录组、ChIP-PCR、mRNA定量和Bloting等技术研究对逆境的应答、转录表达和磷酸化水平；勾画出新的抗逆CgHOG途径模式并揭示其高抗逆的应答调控机理。研究结果将为解决工业菌株选育、绿色发酵技术和耐盐耐旱作物改良中的难题提供新的基因、材料、技术和途径。

The low tolerances to substrate and fermentation products (stress resistance) of the industrial strains are a common problem which has a seriously impact to fermentation industry. In this project, we focus on the high osmolarity glycerol mitogen-activated protein kinase pathway of an independent-property-right hyperosmotic-tolerant industrial yeast. On the basis of whole-genome sequencing, we confirm the genes involved in CgHOG by bioinformatics, and molecular biology methods. Upon stress, the transcription, express or phosphorylation of key genes will be studied by transcriptomics, RT-PCR and bloting technology. A novel stress-resistance response CgHOG pathway and the response regulation mechanism will be performed. In summary, this study provides a series of novel genes, material, technique and pathway in the breeding of industrial strains, green fermentation technology and the crop improvement on salt or drought tolerance.