乳腺癌是全球女性最常见的恶性肿瘤，近年CDK4/6抑制剂在乳腺癌治疗上取得重大进展。鸡血七是土家族经典“七药”和“血药”，能舒筋活血、行气镇痛，对气滞血瘀所致的“乳岩”疗效独特。课题组前期从鸡血七中分离到具有独特新骨架的黄酮及其前体，体外抗乳腺癌细胞增殖显示效果与紫杉醇相当；且其对乳腺癌细胞株CyclinD1-CDK4/6-Rb信号通路相关蛋白有下调作用，但其物质基础和作用机理不明。本研究拟以鸡血七为研究对象，通过乳腺癌细胞株体外增殖模型为活性导向，利用UPLC-Q-TOF-MS和NMR快速识别目标化合物，运用现代分离手段获取鸡血七中活性成分，以抗乳腺癌体内外模型研究鸡血七黄酮的抗癌效果，应用现代生物化学技术对CyclinD1-CDK4/6-Rb信号通路相关蛋白和基因的影响进行检测，揭示其抗乳腺癌作用的物质基础，阐明其抗乳腺癌分子作用机制，为该民族药的临床应用奠定物质理论基础。

Breast cancer is one of the most common malignant tumors in women all over the world. In recent years, the development of CDK4/6 inhibitors have made significant progress in the treatment of breast cancer. Jixue Qi is the classic “Qi” and “Blood” drug of Tujia ethnomedicine and it has unique effects on the mammary cancer caused by qi stagnation and blood stasis. Our group have isolated some flavones with unique new skeleton and their precursors. In vitro anti-breast cancer cells proliferation experimental results revealed that the effect of flavone in Jixue Qi are comparable to the effects of taxol, and pre experiment results showed the flavones in Jixue Qi down-regulate some proteins involved in Cyclin D1-CDK4/6-Rb signaling pathway in breast cancer cell lines, but its pharmacodynamic material basis and mechanism are not clear yet. This study intends to Jixue Qi as the research object, anti proliferation model of breast cancer cell lines guided isolation and rapidly identify the target compounds by UPLC-Q-TOF-MS and NMR were used to discover the active compounds, and advanced isolation methods were used to obtain the active compounds. In vitro and in vivo models of anti-cancer were used to confirm the anti breast cancer effect of Jixue Qi, analyze the expression of genes and proteins involved in D1-CDK4/6-Rb signaling pathway to discover its potential mechanisms of anti-breast cancer effects. this research will contribute to the application of Jixue Qi for anti-breast cancer and lay the material and theoretical foundation for the clinical application of Jixue Qi.