湘西北土家族药物血筒（异型南五味子藤茎）具有祛风除湿、行气止痛之功效，常用于治疗风湿痹症、关节炎等。我们前期研究发现血筒总三萜具有确切的抗炎镇痛和抗关节炎作用，并分离得到了大量结构新颖的三萜内酯类化合物，因此提出三萜内酯类化合物是血筒抗类风湿性关节炎的药效物质基础，并在抗炎免疫相关TNF-α/NF-κB信号转导通路中发挥协同作用的假设。基于此，我们拟用"单-混-谱"药效物质基础筛选模式，研究血筒三萜内酯类化合物单体（"单"）、单体组合（"混"）和有效部位群（"谱"）在TNF-α/NF-κB信号转导通路中的作用靶点、作用环节和作用强度，对阐明血筒抗类风湿关节炎的药效物质基础，揭示血筒"多成分、多靶点、多环节、多途径"的协同作用机理有重要意义，为该民族药治疗风湿痹症的应用奠定物质理论基础，为中药和民族药药效物质基础和作用机理的研究进行有益的探索和尝试。

Xiangxibei Tujia traditional medicine Xue Tong (the stem of Kadsura heteroclite (Roxb) Craib) was recorded to possess the function of expelling wind-evil and removing wetness-evil, regulating qi and alleviating pain in terms of traditional Chinese medicine (TCM) for the treatment of wind-dampness, arthralgia-syndrome and various arthritis.In our previous study, we found that total triterpenoids of Kadsura heteroclite has been recognized for its definite effect in anti-inflammatory, analgesia and anti-rheumatoid arthritis. Moreover, plenty of novel structure compounds of triterpene lactones were identified. Thus, an assumption is made that triterpene lactones are the effective substances of anti-rheumatoid arthritis of Xue Tong, producing a synergistic effect in TNF-α/NF-κB signal tranduction pathway in terms of immune inflammatory reaction in RA. Based on this, for effective substances screening we present a new method called "monomer-mixture-spectrum" mode, aiming at the action target, link, and intensity study of triterpene lactones monomers ("monomer"), monomer combinations ("mixture"), and active frations of Xue Tong ("spectrum") in the TNF-α/NF-κB signal pathway. This project will elucidate the anti-rheumatoid arthritis effective substances of Xue Tong, play an important role in revealing the synergisitic machanism of "multi-component, multi-target, multi-link, multi-approach", provide a substance and theory basis for the treatment of wind-dampness and arthragia-syndrome, carry on beneficial exploration on the effective substances and mechanism study of TCM and ethnomedicine.