长链非编码RNA NONHSAT030515在脂肪干细胞成软骨分化中的作用及机制研究

最近研究发现lncRNAs在干细胞分化中发挥重要作用，但其在hADSCs成软骨分化中的作用及机制还不明确。前期研究通过lncRNA芯片筛发现NONHSAT030515在hADSCs成软骨分化中呈显著高表达，功能分析发现其明显促进软骨相关蛋白Col2A1,Col10A1及AGGRECAN表达。然而NONHSAT030515在hADSCs成软骨分化作用及分子机制尚不清楚。在此基础上，本研究将继续通过体外和体内实验同时验证NONHSAT030515对hADSCs成软骨分化影响，从而确定其在hADSCs成软骨分化中作用，并通过验证NONHSAT030515/miR-490-5p/BMPR2三者之间的表达调控关系以及三者在hADSCs成软骨分化中的功能关系，最终阐明NONHSAT030515在hADSCs成软骨分化中发挥促进作用的具体作用机制，从而为软骨损伤类疾病的治疗提供新的理论依据及治疗。

Recent studies have found that lncRNAs play an important role in stem cell differentiation, but the role and mechanism of lncRNAs in hADSCs remain unclear. Previous studies found that NONHSAT030515 was highly expressed in chondrogenic differentiation of hADSCs through lncRNA chip screening. Functional analysis showed that NONHSAT030515 significantly promoted the expression of cartilage-related proteins (Col2A1, Col10A1 and AGGRECAN). However, the role and molecular mechanism of NONHSAT030515 in chondrogenic differentiation of hADSCs remain unclear. On this basis, this study will continue to verify the effect of NONHSAT030515 on chondrogenic differentiation of hADSCs in vitro and in vivo, so as to provide the role of NONHSAT030515 in chondrogenic differentiation of hADSCs, and verify the expression and regulation relationship between NONHSAT030515/mir-490-5p/ BMPR2 and their functional relationship in chondrogenic differentiation of hADSCs. Finally, it will clarify the softening of NONHSAT030515 in hADSCs. The specific mechanism of osteogenic differentiation plays a promotive role, thus providing a new theoretical basis and therapeutic target for the treatment of cartilage injury diseases.