益气养阴方对骨髓间充质干细胞归巢和心肌修复的影响及机制研究

骨髓间充质干细胞（MSCs）移植治疗缺血性心脏病是目前最具有前景的治疗手段之一，也是干细胞研究领域的热点，然而MSCs移植治疗同样存在细胞存活率低及归巢差等问题，因此，如何提高干细胞移植后的存活及归巢能力是目前亟待解决的难点问题。益气养阴方是我国经典的中药方剂，临床及基础研究显示其治疗缺血性心脏病疗效显著。本项目前期研究表明益气养阴方具有促进细胞存活及迁移的能力，但其分子机制还不明确。因此，在前期工作基础上，本项目拟通过体内体外实验评价益气养阴方通过改善缺血缺氧微环境提高MSCs存活及归巢能力达到心肌修复作用，并对其分子机制进行探讨。本项目的开展不仅有利于提高MSCs移植治疗缺血性心脏病的疗效，而且能为扩大传统中药的临床应用范围提供实验依据。

Mesenchymal stem cells (MSCs) transplantation is a focused and promising cell therapy for ischemic heart disease. However, the low rate of MSCs survival and homing to target tissues and organs limit the therapeutic efficacy. Therefore, the improvement of MSCs survival and homing rate are urgent to be solved. Yiqiyangyin formula, a prescription of traditional Chinese medicine, has showed dramatic therapeutic efficacy on ischemic heart disease in both clinical and basic studies. Our pre-study shows the notable ability of promoting cell survival and migration of Yiqiyangyin formula, while the specific mechanism remains unclarified. Further research of Yiqiyangyin formula will focus on improvement of hypoxic-ischemic microenvironment and the rate of MSCs survival and homing capabilities associated with myocardial repairing, and the molecular mechanism will be discussed. Our study aims at enhancing the curative effect of MSCs transplantation therapy on ischemic heart disease and expanding the scope of clinical application of traditional Chinese medicine.