早产儿视网膜病（ROP）已成为严重危害患儿视功能的一种主要致盲眼病。该病变中血管内皮生长因子（VEGF）、成纤维生长因子（FGF）、胰岛素样生长因子（IGF）等在ROP视网膜新生血管的发生中扮演着重要角色，但具体的发病机制尚不清楚。本研究将针对具有酶活性和非酶活性的heparanase在ROP视网膜新生血管发生中的作用，使用氧诱导视网膜病变模型（OIR模型）和人视网膜血管内皮细胞（HREC）缺氧模型，从体内、体外实验两方面分别通过高、低表达heparanase来研究heparanase与FGF，IGF和VEGF表达之间的相关性，以及不同时间点、不同表达的heparanase对视网膜新生血管的形成的影响及对HREC迁移、增殖和HREC增殖迁移的细胞信号通路的影响，揭示heparanase在ROP视网膜新生血管发生中的基因功能，为临床治疗ROP提供新的理论依据，对防盲治盲具有重要意义。

Retinopathy of premature (ROP) is a major cause of blindness in children that suffer from ROP-related visual impairments. Many molecules are involved in retinal neovascularization of ROP, with vascular endothelial growth factor (VEGF), fibroblast growth factor（FGF), insulin-like growth factor (IGF-1). Nevertheless, the specific mechanism leading to ROP remains unknown. The objectives of this study were to determine the role of heparanase in retinal neovascularization and whether heparanase can become a potential new therapeutic target for ROP. In this study, we investigated enzymatic and nonenzymatic functions of heparanase are involved in retinal neovascularization of ROP, we used an oxygen-induced retinopathy (OIR) mouse model and human retinal vascular endothelial cell (HREC) anoxia model via up-regulation and down-regulation expression level of heparanase to try to determine whether heparanase expression is closely related to VEGF, FGF, IGF-1 expressions in vivo and vitro experiment, and differential time points and differential heparanase expressions can alter retinal neovascularization of OIR and HREC migration and proliferation. We also aimed to determine HREC migration and proliferation correlate with via which signal transduction pathway this action may occur. It will supply some new theories for treatment ROP. It is importantly to the prevention and treatment of blindness.