视网膜中央静脉阻塞（CRVO）是引起视力严重下降的常见疾病。引起CRVO发病的主要原因是视网膜静脉血栓的形成，但具体的发病机制尚不清楚。我们的前期研究提示Heparanase与CRVO的血栓形成密切相关，因此本研究旨在通过高低表达大鼠模型体内的Heparanase后，观察不同组大鼠模型和CRVO患者视网膜血管形态学的改变，及不同组大鼠模型视网膜和CRVO患者静脉血的Heparanase，IL-6，MCP-1，TLR-2，TLR-4，NF-KB，MMP (蛋白水解酶) 家族蛋白，凝血因子，以及蛋白C，蛋白S等的表达与对照组的相关性。研究不同表达的有酶活性的Heparanase与炎症蛋白，MMP的相关性及其细胞信号通路途径和不同表达的非酶活性的Heparanase在改变血液凝血活性中的作用机制。揭示Heparanase在CRVO的血栓形成中的作用机制，为临床上治疗CRVO提供新的治疗思路。

Central retinal vein occlusion (CRVO) is a common diseases cause of severe visual impairment. The main risk factor is the formation of retinal vein thrombosis, but the exact mechanism is unclear. We have known that there is close correlation between the vein thrombosis of CRVO and heparanase. The objectives of this study were to determine the role of heparanase in CRVO and whether heparanase can become a potential new therapeutic target for CRVO. We collected peripheral blood of patients with CRVO, established Wistar rat and spontaneously hypertensive rat model via up-regulation and down-regulation expression level of heparanase to try to determine rat of different groups and the CRVO patients retinal vascular morphological changes and whether heparanase expression is closely related to Il-6, MCP-1, TLR-2, TLR-4 and NF-KB, MMP-2, MMP-9 and MMP-14 as well as the relevance of the expression of coagulation factors, protein C, protein S. In this study, we investigated enzymatic and non-enzymatic functions of heparanase are involved in CRVO, such as promoting plaque inflammatory response, synergy with proteolysis enzymes, and involving in hypercoagulable states, etc, as well as different mechanisms of cell signaling pathways. It is importantly to the prevention and treatment of blindness.