西兰花种子在发芽成苗过程中，硫苷及其水解产物萝卜硫素含量大幅增加。基于萝卜硫素极强的生理活性，提高其转化效率、揭示其转化机理近年成为关注焦点。申请人前期研究发现单独使用HCl或CaCl2电解水能明显提高西兰花芽苗中萝卜硫素含量，但作用机理尚不完全清楚。本项目拟制备CaCl2-HCl复合电解水，考察pH、有效氯浓度、活性氧水平及钙离子协同作用对西兰花苗中萝卜硫素生成的影响，确定影响因子及制备条件。基于多因素调控作用，探究发芽过程中活性氧信号及抗氧化酶活变化，氨基酸代谢途径及钙信号传递变化，萝卜硫素合成关键酶活、上皮硫特蛋白活性，以及相关基因表达。结合代谢组学、蛋白质组学分析，从分子和基因水平揭示CaCl2-HCl复合电解水调控西兰花苗中萝卜硫素的机理。分析西兰花苗营养物质及多酚、黄酮、花青素等活性成分的变化，评价富含萝卜硫素西兰花苗的品质特性。研究成果将为功能性西兰花苗菜食品开发提供支撑。

The contents of glucosinolate and its hydrolyzate sulforaphane increase significantly during the germination of broccoli seeds. Due to the very highly bioactive activity of sulforaphane, the research interests focus on enhancing transformation efficiency from glucosinolates to sulforaphane and revealing its transformation mechanism. Our previous studies found that electrolyzed water prepared by HCl or CaCl2 solutions could promote the accumulation of sulforaphane in the germination of broccoli sprouts, but the mechanism is not fully clear. In this project, the synergy effects of pH, available chlorine concentration, reactive oxygen species and calcium of electrolyzed water prepared by CaCl2-HCl on the formation of sulforaphane in broccoli sprouts would be evaluated, and the impact factors and preparation conditions would be revealed. Based on the multi-factor regulations of CaCl2-HCl electrolyzed water, the changes of reactive oxygen signal, antioxidant enzyme activity, amino acid metabolism pathway, transmission of calcium signal, key enzymes activities and relative gene express in sulforaphane synthesis and epithiospecifer protein activity would be investigated comprehensively during the germination of broccoli seeds. Combined with metabolomics and proteomics analysis, the mechanism of CaCl2-HCl electrolyzed water on improving the contents of sulforaphane in broccoli sprouts during germination would be revealed at molecular and genetic levels. The nutrients and bioactive compounds including polyphenols, flavonoids and anthocyanins will be analyzed. The quality characteristics of broccoli sprouts rich in sulforaphane will be evaluated. The results will provide support for development of functional broccoli sprouts.