GF&SPF&CL级BALB/c小鼠肠道CD4+Foxp3+Treg细胞与微生物群的组成、代谢产物、定植时间密切相关，但肠道Treg来源、分化发育、特征以及与胸腺、脾脏Treg之间的关系远未得到阐明。本项目在已建立的HTS分析CDR3组库平台和预实验基础上，以15天、3月龄、6月龄三个关键时期GF&SPF&CL级BALB/c小鼠为研究对象，采用Illumina HTS技术和系统的微生物与免疫生物信息学分析方法，对比解析其胸腺（中枢）、脾脏（外周）、肠道（区域）组织器官中CD4+Foxp3+Treg细胞与CD4+Foxp3-T细胞TCRβCDR3组库的同质性与异质性（不同时间、空间）,为肠道微生物群对局部和全身T细胞亚群的解析提供系统的对比数据、新的技术方法与思路，为GF&SPF&CL级BALB/c小鼠在实验中的应用提供CD4+Foxp3+Treg细胞时、空异质性的参考数据。

The intestinal CD4+Foxp3+Tregs are closely related with the composition, metabolites and residence location with microbiota of GF&SPF&CL BALB/c mice.But it is still unclear about the differences among CD4+Foxp3+Tregs from intestine, thymus and spleen. In this project, based on the established techniques for sequencing and analyzing T&B cell CDR3 repertoires by high-throughput sequencing and the preliminary experiments. GF（Germ Free）, SPF(Specific pathogen Free) and CL(Clean animal) BALB/c mice mice will be selected at three key point during immune system development (15 days, 3 month and 6 month) , we will analyze the temporal-spatial heterogeneity of thymus(central immune organ), spleen(peripheral immune organ) and intestinal(tissue-specialized immune organ) CD4+Foxp3+Tregs and D4+Foxp3-T cells TCR β CDR3 repertoires using Illumina HTS. To explore the influence of gut microbiota on diversity, clonality, overlapping et al. in CD4+Foxp3+Tregs TCR β CDR3 repertoires. it will provide the basic data for uncover the mechanism of immune tolerance/response to intestinal microbiota, and will provide comparative data, a new way and technology and new ideas for explore the characteristics of tissue-specialized organ T cell subsets in the GF&SPF&CL BALB/c mice.