反胶束生物分离植物蛋白技术不需要大量的酸与碱，具有保持蛋白活性、易规模化、高效节能等优点，越来越受到人们的关注。由于反胶束体系内微环境的特殊性质，改性蛋白的机理尚不清楚，反胶束制备植物蛋白仍处于研发阶段。本项目拟从蛋白亚基的角度，建立萃取大豆7S与11S球蛋白亚基的反胶束体系，通过分子动力学实验研究蛋白分子与反胶束体系的相互作用；系统的研究反胶束体系萃取过程中，7S与11S球蛋白比例变化与亚基结构及亚基功能性之间的相互关系。本项目旨在阐明反胶束萃取过程中7S与11S球蛋白比例变化的机理，为促进反胶束萃取蛋白的理论发展和开发高品质的蛋白产品奠定理论和技术基础。

Because the method of separation proteins by reverse micelles has many advantages, such as the less acid and alkali solution, low cost, maintaining protein activity and being easy to scaleup, it is very interesting for many researchers. However, the mechanism of modified proteins is not clear in reverse micelle environment, which still is under development. The work intends to protein subunit angle, establishing the reverse micelle systems of extraction 7S and 11S globulin subunit from soybean. The ration change of 7S and 11S globulins, subunit structure and functional properties were studied by interaction of protein molecule and reverse micelles. The objective of this study was to explain the change mechanism of 7S and 11S globulin ration, which lays the theoretical and technical basis for promoting the reversed micellar extraction of protein and the development of high-quality protein products.