早期筛查技术的运用和HPV疫苗的使用使宫颈癌预后有所提高，但复发性或转移性的宫颈癌患者预后仍然较差。因此，寻找有效的治疗靶点对提高治疗效果具有重要的意义。有报道显示癌蛋白Skp2在宫颈癌中是高表达，但是Skp2在宫颈癌中的作用机制依然不清楚。我们的前期研究中初步发现Skp2过表达后促进了宫颈癌细胞的生长，抑制了细胞凋亡。预实验还表明在过表达Skp2的宫颈癌细胞内，CBX7蛋白表达下调。另外，过表达CBX7的宫颈癌细胞，细胞的增殖能力减弱，提示CBX7可能在宫颈癌中是一个抑癌基因。因此我们推测Skp2可能通过降解CBX7的表达而发挥癌基因的功能。本课题旨在探索Skp2和CBX7在宫颈癌中的功能和Skp2是否靶向降解CBX7表达，以及化合物Rottlerin是否通过Skp2/CBX7通路来抑制宫颈癌的生长、转移，发挥抗宫颈癌的作用。我们的研究可能为开辟宫颈癌靶向治疗的新途径提供理论和实验依据。

Cervical cancer is one of common malignancies in women. Although screen detection for early diagnosis of cervical cancer and HPV vaccine for prevention has been used, the patients with reoccurred or metastatic cervical cancer have poor survival rate. Therefore, it is important to discover the new therapeutic targets to improve the treatment benefit and survival rate. It has been known that oncoprotein Skp2 plays a critical role in the tumorigenesis. Studies have demonstrated that Skp2 is highly expressed in cervical cancer, however; the mechanism is elusive. Our preliminary results showed that over-expression of Skp2 promoted cell growth and inhibited cell apoptosis in cervical cancer cells, suggesting that Skp2 could be an oncoprotein in cervical cancer. We also found that CBX7 is down-regulated in Skp2-overexpressing cervical cells. Moreover, over-expression of CBX7 inhibited cell growth in cervical cancer cells, indicating that CBX7 could be a tumor suppressor. We hypothesize that Skp2 exerts its oncogenic function via inhibition of CBX7. Therefore, we aim to determine the function of Skp2 and CBX7 in cervical cancer. We also explore whether and how Skp2 regulates CBX7 expression and whether natural compound Rottlerin exhibits anti-tumor activity due to regulation of Skp2/CBX7 pathway. Our study could provide the molecular evidence for the treatment of cervical cancer.