脂肪分解代谢伴随着猪卵母细胞成熟全过程。申请者前期结果显示，上调脂肪分解作用可增加脂肪酸的释放及ATP的生产，为猪卵母细胞体外成熟提供能量来源。但仅从能量途径不足以揭示脂肪分解代谢与卵母细胞成熟之间的相互作用，非能量途径所涉及的脂肪分解代谢物也参与调控多种生物学事件，而这些代谢物是否调控以及如何调控猪卵母细胞体外成熟，都尚不清晰。据此，本研究将特异性敲低脂肪分解酶HSL的表达，推断其对猪卵母细胞体外成熟的生物学作用；通过添加脂肪分解代谢产物对被阻断的脂肪分解活动进行拯救，追踪卵母细胞发育相关标志物动态变化及卵母细胞和胚胎发育情况，筛选出参与猪卵母细胞体外成熟的关键脂肪分解功能性代谢物；通过Real-time PCR、免疫荧光、蛋白印迹等方法，探索关键脂肪分解功能性代谢物在猪卵母细胞体外成熟过程中的作用机制。该研究将会为提高猪卵母细胞体外成熟质量提供理论支持。

The lipolytic metabolism accompanies the whole porcine oocyte maturation process. In the previous study, we demonstrated that the up-regulation of lipolytic activity could increase fatty acids and ATP contents, and provide energy sources to the porcine oocyte in vitro maturation. However, it is not enough to reveal the interaction between lipolytic metabolism and porcine oocyte maturation. The lipolytic metabolites are also involved in regulating various biological events with the non-energy pathway, but it is not clear whether the lipolytic metabolites regulate porcine oocyte maturation and how is it regulated. Firstly, to understand the biological role of HSL in the porcine oocyte, we design specific HSL down-regulation systems for blocking HSL activity. Secondly, to screen the key lipolytic metabolism-derived functional molecule(s), we supplement lipolytic metabolites to porcine IVM medium after HSL down-regulation, and analysis the development-related markers, oocyte maturation and subsequent embryonic development competence. Finally，we study the mechanism of key lipolytic metabolism-derived functional molecule(s) during porcine oocyte in vitro maturation through Real-time PCR, immunofluorescence and western blot, etc. The project solution will provide theoretical support for improving the quality of porcine oocyte in vitro maturation.