新型组合多孔材料方法及其在结肠癌质谱检测分析中的应用

具有不同孔径尺寸、表面化学的多孔材料能够根据孔道与客体生物分子的尺寸匹配效应，以功能化设计的表面相互作用为驱动力，选择性地从生物样品中提取分析相应的分子组，有效提高分子质谱检测鉴定效率。然而，现阶段组合多孔材料的方法模型极少，研究通常使用单一结构/功能的多孔材料提取分析特定类别的蛋白质/多肽分子，不利于获取生物样品的完整信息，在实际应用中极为受限。本项目拟在生物系统的分子组成分析中引入新型组合多孔材料方法，即按照一定的顺序组合使用不同孔径、表面化学的多孔材料，程序化分析待测样品的蛋白质组和多肽组，筛查蛋白质/多肽分子标志物及其后翻译修饰作用，其主要包含以下三方面内容：一）功能化有序大孔材料（孔径约为100 nm）及其在蛋白质组检测分析中的应用；二）功能化有序介孔材料（孔径小于10 nm）及其在多肽组检测分析中的应用；三）组合使用以上大孔、介孔材料用于检测分析实际结肠癌生物样品的分子组成。

The detection efficacy can be highly enhanced using nanoporous materials during bio-molecular profiling by mass spectrometry. Nanoporous materials with specific pore size can selectively extract bio-molecules due to the size-selective effect, where designed surface chemistry is essential to provide the desirable driving force. However, there are very few reports on the extraction and detection of bio-molecules using a combination of different materials and most current strategies are focused on the application of a single type of nanoporous materials. Consequently, only parts of bio-molecules can be profiled and the whole bio-system is not well understood. In this project, we aim to build a platform technology based on novel nanoporous materials and their combinations, superior to conventional methods and commercial products. The combo-pore technology can enable advanced profiling towards diverse proteins/peptides and investigate the post-translational modifications of these molecules in a programmable manner. There are three main aspects to be included in this project: 1) functional macroporous materials (pore size around 100 nm) for proteins proteolysis and identification in proteomic study; 2) functional mesoporous materials (pore size less than 10 nm) for peptides extraction and detection in peptidomic study; 3) combinations of macro-/meso-porous materials with designed surface chemistry towards disease biomarkers screening and comprehensive bio-molecular profiling in colon cancer bio-samples.