MicroRNA对板栗疫病菌毒力的调控机制研究

低毒病毒侵染可导致板栗疫病菌（Cp）表型发生改变并显著降低真菌的毒力，因而该系统被视为解析病原真菌致病机理的一个优秀模型。MicroRNA(miRNA)是进化保守的非编码小分子RNA，与生物体的生长、发育、代谢及疾病的发生密切相关。然而，miRNA在病原真菌中的研究却很少。本课题拟以Cp野生强毒菌株和受病毒侵染的弱毒菌株为实验材料，通过Solexa深度测序，获得板栗疫病菌的miRNA图谱，寻找差异表达的miRNA。对差异表达的miRNA进行过表达/抑制表达，研究其与真菌毒力的关系，进一步鉴定其作用靶标，并研究靶标基因对毒力的作用，明确关键miRNA在真菌毒力和重要生理性状表达上的作用，从而进一步揭示Cp毒力调节的机制。

Hypovirus/Cryphonectria parasitica (Cp) system is regarded as an excellent experimental system to dissect the virulence regulation mechanism, since infection by a hypovirus can profoundly reduce the virulence and result in persistent changes in multiple traits of this pathogenic fungus. MicroRNA (miRNA) are a class of endogenous non-coding RNAs that play an important role in many biological processes，such as growth，development，metabolism and diseases. To date, little is known about the role of miRNA in pathogenic fungus. In this study, the miRNA from the EP155 and EP713 will be identified and compared with Solexa depth sequencing analysis. The differentially expressed miRNA will be to study the relationship between miRNA and the virulence by up-regulating/down-regulating its expression. To elucidate the regulatory mechanism of miRNA in the fungus virulence, the targets of miRNA will be identified and the role of target protein in virulence will be investigated.The role of key miRNA will be investigated physiologically and pathogenetically. The results obtained will help to unvail the mechanism of virulence regulation in Cp.