脂肪肝是严重影响人类健康的代谢疾病，其致病的分子机制还不是十分清楚。鹅肝脏沉积脂肪能力很强，它不仅在形成脂肪肝（肥肝）后可以恢复，而且没有明显的病理症状，是人类脂肪肝研究理想的动物模型。本项目以朗德鹅为素材，人工填饲高能饲料，诱发其产生脂肪肝，采用第二代高通量测序方法，检测鹅肥肝形成过程中miRNA的表达情况，结合本课题组前期鹅肥肝转录组学等研究结果及生物信息学方法预测和筛选出与鹅肥肝形成有重要关联的miRNA及其靶基因和代谢途径，在此基础上，在鹅细胞水平上，采用miRNA过表达或干扰方法，了解目标基因表达及对脂类代谢的影响。本研究旨在以鹅为动物模型，以转录组学研究为基础，了解鹅肥肝形成过程中miRNA的表达及其与基因间的网络调控，为人类脂肪肝研究提供有益的参考。

Fatty liver disease is a metabolic disease which affects human health seriously. However the molecular mechanisms of pathogenesis are still unclear. Goose has a natural ability to store lipid in its liver, without any obvious pathological symptoms when recovered, which provides an ideal model for studying on fatty liver. In this project, Landes will be overfed with a carbohydrate-rich diet to induce the fatty liver. Next-generation sequencing technologies will be used to detect the expression of miRNAs through fatty liver formation. Combine with previous studies of goose fatty liver transcriptomics and some bioinformatics methods, the miRNAs and their target genes and the metabolic pathways which have important relationships with goose fatty liver will be identified. And on this basis, miRNAs over-expression and interference in goose liver cells will be used to understand their effect to the target genes and fat metabolism. In this project, based on the transcriptomics research, goose will be chosen as the animal model to study the miRNAs expression during the formation of fatty liver and the network between miRNAs and genes. This can provide useful evidence to help expanding knowledge in human fatty liver research.