甲状腺癌是目前发病率上升最快的恶性肿瘤之一，其中80%~85%为乳头状癌(PTC)。PTC是否存在过度诊疗是目前争议的焦点，而解决该问题的核心是准确评估PTC病灶是否具有侵袭性生物学行为特征。我们在前期研究中通过检测转移和非转移PTC组织标本，筛选出差异基因丙酮酸羧化酶(PC)基因。已知PC是肿瘤代谢重编程中的关键酶之一，与部分肿瘤的侵袭性生物学行为关系密切。我们初步研究发现PC基因表达水平可影响甲状腺癌的侵袭性生物学行为。此外，有研究报道PC基因与Wnt/β-catenin信号通路及上皮-间质转变(EMT)密切相关。为进一步探讨PC基因对PTC侵袭性生物学行为影响的分子机制，我们拟在前期研究基础上进一步探讨PC基因对PTC代谢重编程及侵袭性生物学行为的作用，及Wnt/β-catenin信号通路与EMT相互作用调控PC表达的潜在分子机制，为PC基因作为PTC精准诊疗的分子靶点提供理论基础。

Over treatment is the major concern in the clinical management of thyroid cancer, particularly papillary thyroid cancer (PTC) which constitutes 80~95% of cases and witnesses rapidly increased morbidity recently. The precision treatment relies on the accurately determined aggressiveness of tumor lesion. The preliminary microarray study on differential expression of multiple candidate genes revealed that aberrant expression of pyruvate carboxylase (PC) gene was associated with metastasis of PTC. The PC is a key enzyme for metabolic reprograming and closely associated with aggressive biological behavior of cancer cells. It has been reported that PC gene might exerted its biological effect via Wnt/β-catenin signaling pathway and deeply involved in epithelial-mesenchymal transition (EMT). The proposed study will explore the molecular mechanism underlying PC gene regulated metabolic reprograming and aggressive biological behavior of PTC based on comprehensive study on the effect of PC gene upon Wnt/β-catenin signaling pathway and EMT. The clarification of the role of PC gene in the biological behavior of PTC provides a novel target for precision treatment of PTC.