阿尔茨海默病（AD）是最常见的老年期痴呆，其确切的发病机制仍不清楚。Aβ神经毒性在其发病过程中起着关键性作用，课题组前期研究提示mRNA-代谢网络紊乱是Aβ神经毒性的主要表现。补肾填髓法是中医治疗AD的基本治法，具有多成分、多靶点、多途径整体协同的作用特点。基于前期研究结果，我们提出假设：补肾填髓法能通过调控mRNA-代谢网络，拮抗Aβ神经毒性，发挥其治疗AD作用。本项目拟以补肾填髓方为实验工具，通过整合运用各种组学和生物信息学方法，捕获补肾填髓法拮抗Aβ神经毒性网络防治AD的靶标分子群，构建转录组-代谢组网络图谱，最终结合体内-外实验，采用分子生物学方法验证并发现网络调控系统关键节点及其在疾病发生发展过程中的结构与功能定位，以期全面、系统地阐释补肾填髓法靶向拮抗Aβ神经毒性防治AD的调控机制。本项目将为中医药治疗AD提供新的证据和思路，同时也为中医治则治法研究提供全新的策略和方法。

Alzheimer's disease (AD) is the most common form of dementia in the elderly. The exact etiology and pathogenesis of Alzheimer's disease (AD) still remains unclear. But accumulating evidence indicates that the neurotoxicity of Aβ play a critical role in the disease progress of AD. We previously showed that Aβ could cause the alterations of mRNA- metabolomic network of the AD. The Bushen Tiansui therapy is the TCM major therapeutic method for AD, and Bushen Tiansui formula could treat AD in a multi-component, multi-target, multi-pathway manner. Hence, we proposed a hypothesis that BSTSF is a promising therapeutic agent that exerts effects at multiple sites within the mRNA- metabolomic network induced in AD. To confirm the hypothesis, we integrate transcriptomics, metabolomics and bioinformatic methods to capture the key molecular clusters referred to mRNA- metabolomic networks after BSTSF treatment following AD; to apply bioinformatics analysis methods and mathematical modeling approaches to conduct the related mRNA-metabolomic networks; and to verify the above network map according to in vivo and in vitro studies. Finally, we intend to delineate the regulatory mechanism of Bushen Tiansui therapy for treating Alzheimer's disease by targeting the neurotoxicity of Aβ. This plan will provide the new evidences for the treating AD by Bushen Tiansui therapy, and provide new ideas for the research of TCM therapeutic principles and methods.