灰霉病是设施黄瓜常见病害，但黄瓜防御灰霉菌侵染的调控机制还不清楚。CC型谷氧还蛋白基因CsGRX是植物应对灰霉菌侵染的关键调控因子，我们发现，黄瓜CC型CsGRX1基因响应灰霉菌侵染、过表达使转基因株感病，推测其在灰霉病抗性反应中起重要的负调控作用，但其机理尚不明确。本项目拟以黄瓜抗灰霉病种质14-1为试材，通过定量PCR分析各CC型CsGRXs对灰霉病的响应、原生质体暂态表达分析各CsGRXs的作用、酵母双杂交分析CsGRX1在灰霉侵染过程中的互作蛋白、CsGRX1基因关键酶活性位点及ALWL结构域突变研究对基因功能的影响、用CsGRX1基因对拟南芥grxs13-1突变体进行互补及CsGRX1基因过表达和抑制表达对黄瓜进行遗传转化，研究CsGRX1对灰霉病抗病性、氧化还原状态、JA含量及JA通路基因表达的影响，阐明CsGRX1负调控灰霉病抗性反应的机制，为改良黄瓜灰霉病抗病性奠定基础。

The Botrytis cinerea is one of the common diseases under the protect cultivation, and the molecular mechanism of defense response in cucumber to Botrytis cinerea was not clear. The CC-type glutaredoxin was the key regulator to the infection of Botrytis cinerea. It was found that the expression of CC type CsGRX1 in cucumber was responsive to the infection of Botrytis cinerea, and the overexpression of CsGRX1 in Arabidopsis was more susceptible to Botrytis cinerea. It was assumed that CsGRX1 was very one of important negative regulators to the infection of Botrytis cinerea. Up to now, we didn’t know the mechanism of how CsGRX1 regulated the response to Botrytis cinerea. The expression pattern of CsGRXs using Real-time PCR, and the suppression function of CsGRXs to the JA signal pathway using transient protoplast expression system and interaction protein analyzing using Yeast-Hybrid system were investigated in 14-1 cucumber seedlings during infection of Botrytis cinerea. The enzyme activity site and ALWL motif of CsGRX1 were mutated respectively and the function of mutated gene was analyzed. The overexpression vector, RNAi vector will be constructed using the gateway technology and then they will be transformated into the cucumber inbred line 14-1 and Arabidopsis respectively. The complementation experiments using grxs13-1 knocked down Arabidopsis was carried out to identified the function of CsGRX1, and the disease resistance, the redox status change and the effects to the JA signal pathway were analyzed to elucidate it's negative regulation mechanism to Botrytis cinerea, which will put the basis of understanding resistance response of cucumber to Botrytis cinerea.