Identification and expression analyzes of CC-type glutaredoxin in cucumber (Cucumis sativus L.) under abiotic stress
Identification and expression analyzes of CC-type glutaredoxin in cucumber (Cucumis sativus L.) under abiotic stress
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非生物胁迫下黄瓜CC型谷氧还蛋白的鉴定及表达分析
DOI：
10.1016/j.scienta.2021.110417
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发表时间：
2021-11
影响因子：
4.3
通讯作者：
Chen Shuxia
中科院分区：
文献类型：
--
作者：
Yang Yuting;Xue Wanyu;Chen Panpan;Yuan Xin;Li Xvzhen;Zhang Tingting;Chen Shuxia
谷氧还蛋白（GRX）是细胞氧化还原状态的关键调节因子，在植物生长发育和胁迫反应中起着关键作用。在黄瓜中共发现45个GRX基因，其中16个属于CC型。根据我们的生物信息学分析结果，这些CC型GRX在黄瓜、拟南芥和水稻中高度保守。黄瓜CC型GRX（CsGRX）基因在植物不同器官中的表达量不同，水杨酸（SA）处理诱导CsGRX 1、CsGRX 5、CsGRX 9、CsGRX 13和CsGRX 16在3 ~ 6 h表达，茉莉酸甲酯（MeJA）处理诱导CsGRX 1、CsGRX 3、CsGRX 8、CsGRX 13、CsGRX 14和CsGRX 17在3 ~ 12 h表达。MeJA处理在0 ~ 24 h诱导CsGRX 11表达，而CsGRX 3和CsGRX 11在42 °C处理的早期和甲基紫精处理的后期诱导表达; PEG和NaCl处理诱导CsGRX 4、CsGRX 6、CsGRX 7、CsGRX 13和CsGRX 11表达。这些结果表明GRX基因在应激反应中具有多种功能。结果表明，CsGRX-绿色荧光蛋白（GFP）融合蛋白的亚细胞定位结果表明，部分同源基因定位于细胞核; CsGRX 4在拟南芥中异源表达，其转基因植株表现出盐敏感性，在盐胁迫下种子萌发率和根长均低于野生型。本研究结果为黄瓜GRX基因的进一步研究奠定了基础。
Glutaredoxins (GRXs) are key regulators in the cellular redox state and play pivotal roles in both plant development and responses to stress stimuli. Here, we found 45GRXgene members in cucumber, 16 of which were classified as CC-type. These CC-typeGRXs were shown to be highly conserved among cucumber,Arabidopsis thaliana, and rice, according to our bioinformatic analyzes results. Cucumber CC-typeGRX(CsGRX) genes showed varying expression levels in different plant organs and in response to different abiotic stresses.CsGRX1, CsGRX5, CsGRX9, CsGRX13, andCsGRX16expression was induced from 3 to 6 h by salicylic acid (SA) treatments, whereasCsGRX1, CsGRX3, CsGRX8, CsGRX13, CsGRX14, andCsGRX17expression was induced from 3 to 12 h with methyl jasmonate (MeJA) treatments. Moreover,CsGRX11expression was strongly induced from 0 to 24 h by MeJA treatment, whereasCsGRX3andCsGRX11expression was strongly induced at the early stages of 42 °C treatments and at the later stages of the methyl viologen treatments.CsGRX4, CsGRX6, CsGRX7, CsGRX13andCsGRX11expression was induced by PEG and NaCl treatments. These results indicated the versatile roles ofGRXgenes in response to stress stimuli. The subcellular localization results for threeCsGRX–green fluorescent protein (GFP) fusion proteins indicated that some orthologs were localized to the nucleus.CsGRX4that responded to salt stress were heterologously expressed inArabidopsis, and its transgenic lines demonstrated salt sensitivity, showing lower seed germination rate and shorter root length than their wild type counterparts under salt stress. Our results provide a foundation for future studies onGRXgenes in cucumber.
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影响因子：
5.6
作者：
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通讯作者：
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影响因子：
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影响因子：
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DOI：
10.1186/s12284-016-0105-6
发表时间：
2016-12
期刊：
Rice (New York, N.Y.)
影响因子：
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作者：
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影响因子：
13.3
作者：
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通讯作者：
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