Redox Status, JA and ET Signaling Pathway Regulating Responses to Botrytis cinerea Infection Between the Resistant Cucumber Genotype and Its Susceptible Mutant.
Redox Status, JA and ET Signaling Pathway Regulating Responses to Botrytis cinerea Infection Between the Resistant Cucumber Genotype and Its Susceptible Mutant.
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DOI：
10.3389/fpls.2020.559070
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发表时间：
2020
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Yang Y;Wang X;Chen P;Zhou K;Xue W;Abid K;Chen S
灰霉病菌是一种重要的坏死性真菌病原菌，寄主范围广，能给黄瓜造成巨大的经济损失。然而，黄瓜对该病原菌的抗性机制尚不清楚。本研究对抗病突变体26号及其敏感突变体26M侵染灰霉病菌后的孢子生长进行了显微镜观察、氧化还原状态测定和转录组分析。DAB和NBT染色结果表明，26号菌丝较短，孢子萌发率较低，对H_2O_2、O_2~-的促进作用较小，总谷胱甘肽含量(GSH+GSSG)较低。转录组数据显示，病原菌侵染后，26号和26M分别鉴定出3901个和789个差异表达基因。这些DEG高度富含氧化还原调节途径、激素信号途径和植物-病原菌相互作用途径。在26号中，谷胱甘肽S转移酶基因、可能的过氧化物酶基因和NADPH氧化酶基因表达上调，而在26M中这些基因的表达变化不大。与26M相比，26号在侵染后茉莉酸和乙烯的生物合成和信号通路被显著激活。病原菌侵染26号比26号诱导了更多的植物防御相关基因，包括有丝分裂原激活蛋白激酶、钙调蛋白、类钙调素蛋白、钙依赖蛋白激酶和WRKY转录因子。最后，建立了黄瓜灰霉病菌侵染后抗病突变体的抗性差异模型。
Botrytis cinerea is an important necrotrophic fungal pathogen with a broad host range and the ability to causing great economic losses in cucumber. However, the resistance mechanism against this pathogen in cucumber was not well understood. In this study, the microscopic observation of the spore growth, redox status measurements and transcriptome analysis were carried out after Botrytis cinerea infection in the resistant genotype No.26 and its susceptible mutant 26M. Results revealed shorter hypha, lower rate of spore germination, less acceleration of H2O2, O2 -, and lower total glutathione content (GSH+GSSG) in No.26 than that in 26M, which were identified by the staining result of DAB and NBT. Transcriptome data showed that after pathogen infection, a total of 3901 and 789 different expression genes (DEGs) were identified in No.26 and 26M respectively. These DEGs were highly enriched in redox regulation pathway, hormone signaling pathway and plant-pathogen interaction pathway. The glutathione S-transferase genes, putative peroxidase gene, and NADPH oxidase were up-regulated in No.26 whereas these genes changed little in 26M after Botrytis cinerea infection. Jasmonic acid and ethylene biosynthesis and signaling pathways were distinctively activated in No.26 comparing with 26M upon infection. Much more plant defense related genes including mitogen-activated protein kinases, calmodulin, calmodulin-like protein, calcium-dependent protein kinase, and WRKY transcription factor were induced in No.26 than 26M after pathogen infection. Finally, a model was established which elucidated the resistance difference between resistant cucumber genotype and susceptible mutant after B. cinerea infection.
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影响因子：
7.4
作者：
Audenaert, K;De Meyer, GB;Höfte, MM
通讯作者：
Höfte, MM
影响因子：
3.5
作者：
Chagué, V;Danit, LV;Sharon, A
通讯作者：
Sharon, A
影响因子：
7.2
作者：
Chassot, Celine;Buchala, Antony;Lamotte, Olivier
通讯作者：
Lamotte, Olivier
影响因子：
5.6
作者：
Dey S;Corina Vlot A
通讯作者：
Corina Vlot A
影响因子：
2.9
作者：
Ghanta, Srijani;Bhattacharyya, Dipto;Chattopadhyay, Sharmila
通讯作者：
Chattopadhyay, Sharmila