miRNA、长链非编码RNA(lncRNA)调控肿瘤侵袭转移。我们前期发现：miR-199b和lnc-AC012313.1-1与结直肠癌肝转移相关；miR-199b负性调控lnc-AC012313.1-1；形成RNA诱导沉默复合体（RISC）可能为重要机制。本课题将明确miR-199b通过调控lnc-AC012313.1-1影响结肠癌侵袭转移；验证miR-199b与lnc-AC012313.1-1序列特异性结合；明确miR-199b通过Ago2蛋白形成RISC参与lnc-AC012313.1-1调控；探索Ago2磷酸化在 miR-199b调控lnc-AC012313.1-1表达的作用；动物体内验证miR-199b通过调控lnc-AC012313.1-1参与结直肠癌肝转移。本课题拟阐明miR-199b通过lnc-AC012313.1-1调控结直肠癌肝转移的作用机制，希望为治疗提供新靶点。

Tumor metastasis could be regulated by miRNAs and long non-coding RNAs (lncRNAs). Previously we have found that both miR-199b and lnc-AC012313.1-1 have a close relationship with liver metastasis of colorectal cancer (CRC); lnc-AC012313.1-1 could be negatively regulated by miR-199b; RNA-induced silencing complex (RISC) may be a key mechanism during this process. This study plans to elucidate the regulative mechanism between lnc-AC012313.1-1 and miR-199b during the the liver metastasis process of CRC; We will prove the sequence specific targeting between miR-199b and lnc-AC012313.1-1, and that the regulation of lnc-AC012313.1-1 by miR-199bis through the RISC, with Ago2 as its core protein. What’s more, phosphorylation of Ago2 during this process will be studied. Finally, we will verify that it is through the regulation of lnc-AC012313.1-1 that miR-199b participate in the liver metastasis of CRC. This study will provide us with the regulation mechanisms between miR-199b and lnc-AC012313.1-1, so as to providing new therapeutic targets for the treatment of colorectal cancer.