Hedgehog信号通路在进化上高度保守，Hh信号在胚胎发育、器官形成和成体稳态维持等过程中发挥了重要的作用；异常的Hh信号活性跟多种人类疾病相关，如发育异常和癌症。Ci及其同源物Gli是Hh信号通路目前已知唯一的转录因子，Ci的调节在Hh信号转导调控中具有非常重要的意义。在本研究中，我们使用果蝇作为模式生物，揭示出激酶Fu对Hh信号调控具有双向性：Fu与泛素连接酶Su(dx)形成复合物，促进Ci的泛素化修饰和蛋白质降解，从而抑制Hh信号。在果蝇翅膀原基发育过程中，Fu/Su(dx)复合物在特定发育组织部位和特定发育时期，精确控制了Hh信号通路的开关状态转变。本项研究将加深我们对Hh信号转导严谨性调控机制的理解。

Hedgehog（Hh）signaling pathway is highly conserved between invertebrates and vertebrates during evolution, Hh signaling plays important roles in embryonic development, organogenesis and homeostasis of adult tissues. Malfunction of Hh signaling lead to human diseases, such as developmental deformity and cancer. As the only known transcriptional factor of Hh signaling pathway, Ci and its orthologs Gli are very important in Hh pathway, and need to be tightly regulated. By using Drosophila as our research model animal, in this study we identified that kinase Fu plays bidirectional roles in Hh signaling：Fu and E3 ligase Su(dx) form a complex to promote the ubiquitination and degradation of Ci, thus precisely control the on-off state shift of Hh signaling, both in a developmental position specific and in a developmental stage specific manner in drosophila wing development. This research will evidently expand our knowledge of how Hh signaling is precisely regulated in development.