近年来介孔二氧化硅纳米材料以独特的药物可控性释放和作为安全的基因载体，引起了肿瘤研究领域的特别关注。本研究在前期实验基础上，预制备叶酸化-温度、PH双响应智能化介孔二氧化硅纳米粒作为抗癌药物/基因载体，共载姜黄素/siRNA-ABCG2，在温敏及 pH 敏感两亲型聚合物调控下实现药物在癌细胞内高效控释，继而联合化疗药物（顺铂、多西紫杉醇、5-FU）进行体内外实验，通过检测不同治疗组喉癌干细胞及荷瘤裸鼠肿瘤抑制率及肿瘤组织中癌细胞的凋亡状态证实共载姜黄素/siRNA-ABCG2组通过双靶向抑制ABCG2的表达逆转喉癌干细胞的MDR现象，稳定释放姜黄素可以起到化疗药物增敏作用最终有效诱导喉癌细胞凋亡，并检测细胞内P13K/AKT、Wnt等多条凋亡信号通路的变化，探索其诱导喉癌干细胞凋亡的机制。该生物载体具有双靶向性，将为喉癌的治疗带来安全的方法，具有潜在的临床应用价值和前景。

In recent years, mesoporous silica nanometer materials with unique drug loading and controlled release characteristics has caused the special attention in cancer research field. This research on the base of the high expression folic acid receptor on the surface in laryngeal cancer cell and the acid environment within the cancer cells as the theoretical support point to producting FA- PH sensitive amphiphilic type mesoporous silica nanoparticles as anti-cancer drug controlled release/gene delivery carrier. In our study, on the basis of preliminary experiment, folic acidification - PH sensitive type amphiphilic mesoporous silica nanoparticles will be made as an anti-cancer drug/gene carrier(curcumin/siRNA - ABCG2 ).Grafted on the surface of PH sensitive type amphiphilic polymer can achieve the effect of PH control release and the drug within a acid environment of the cancer cells can be quickly released. We use this total load of curcumin/siRNA - ABCG2 carrier combined chemotherapy drugs (cisplatin, docetaxel, 5 - FU) to carry on the experiments in vivo and in vitro.By testing different treatment group laryngeal cancer stem cells and a tumor-burdened nude mice tumor inhibition and apoptosis of cancer cells in tumor tissue state confirmed a total load of curcumin/siRNA - ABCG2 group reverse MDR phenomenon of laryngeal cancer stem cells by inhibiting the expression of ABCG2 and the stable releasing of curcumin can have the sensitization effect of chemotherapy drugs which can eventually effectively induce laryngeal cancer cells apoptosis.In the process of experiment we also detect intracellular P13K/AKT、Wnt apoptosis signaling pathways to explore the mechanism of apoptosis induction of laryngeal cancer stem cells.The biological carrier has double targeting property which will bring new and safe method for the treatment of laryngeal cancer and has a broad clinical application value and unique advantage.