帕金森病(Parkinson’s disease, PD)是一种慢性的神经干细胞(Neural Stem Cells, NSCs)分化缺陷，并受到体内微环境的影响。中医理论认为肾虚血瘀是PD的病理基础，故应以补肾活血法治疗。NSCs生存的微环境（即神经干细胞龛/Niche）对NSCs的增殖与分化具有重要的调节作用。前期研究发现，补肾活血复方中药能促进干细胞Niche内NSCs的增殖、并分化为神经元细胞，减少凋亡，为PD脑损伤提供修复。本课题拟以Wnt/Nurr1信号通路为切入点，构建PD大鼠模型，采用行为学分析、RT-PCR、WB、免疫共沉淀等技术手段，揭示补肾活血复方改善帕金森鼠海马区干细胞龛微环境，使NSCs自我更新、增殖与分化达到动态平衡的分子机制，为PD补肾活血中药的研究和开发提供部分科学依据。

Parkinson's disease (PD) has been proved to be a kind of chronic Neural Stem Cells (NSCs) differentiation defect, which is affected by the microenvironment in vivo. The Traditional Chinese Medicine considers that kidney deficiency and blood stasis are the pathological basis of PD. The NSCs Niche plays an important role in regulating the proliferation and differentiation of NSCs. Previous studies have found that the tonifying kidney and promoting blood recipe can promote the proliferation and differentiateof NSCs, reduce apoptosis, and provide repair for PD brain injury. This research propose Wnt/Nurr1 signaling pathway as the key point, in combination with PD rat model constructing, the behavior analysis, RT-PCR, WB, Co-IP ways, to expore the tonifying kidney and promoting blood recipe improve the PD rat hippocampus stem cell niche microenvironment, and make NSCs self-renew、proliferation and differentiation to achieve dynamic balance, provides part of scientific basis for the PD treatment of tonifying kidney and promoting blood.