兽药残留是食品安全领域重点研究课题之一，动物源食品中兽药残留检测是评价食品卫生的重要依据。已报道的检测方法存在仪器昂贵、成本高、费时等缺陷，难于普及和满足市场监督需求。前期研究发现，抗生素类、激素及β-兴奋剂类兽药对Ni(IV)、Ag(III)、Cu(III)过渡金属超常氧化态配合物（UOTMC）氧化鲁米诺发光有增敏作用，据此建立价廉、快速兽药残留卫生检测新体系，但氧化规律和增敏机制尚不清楚。本课题拟研究:①通过测定UOTMC对鲁米诺及各兽药残留目标物的反应过程活化能和动力学参数，探讨UOTMC的氧化规律，并通过该氧化体系的图谱解析和反应产物的鉴定，揭示兽药对UOTMC-鲁米诺化学发光体系的增敏机制；②基于UOTMC-鲁米诺化学发光新体系建立FI-CL检测和毛细管电泳同时分离检测兽药残留系列新方法；③评价新建检测方法的性能指标，为新建方法应用于动物源食品中兽药残留检测提供可靠的方法学基础。

The detection of veterinary drug residues, one of the critical research areas in food safety, is an important base for the evaluations of food health. Previously reported methods have some drawbacks including expensive instrumentation, high-cost and time-consuming; they are difficult to be widely used and to meet the market need of surveillances. In our preliminary studies, we found that some unusual oxidation states of transition metal complexes (UOTMC), e.g. Ni(IV), Ag(III) and Cu(III) complexes, gave rise to oxidation chemiluminescence (CL) when they reacted with luminol and moreover, antibiotics, hormones and analeptics could enhance the CL drastically. Based on these findings, it is very likely that we can establish novel detection systems (referred to: UOTMC-CL) which are anticipated to be rapid and low-cost. The oxidation characteristics and reaction mechanisms of the enhancement are, however, poorly understood. The aims of the present project are: (1) to derive the reaction mechanisms of CL enhancement or inhibition of the UOTMC-Luminol CL systems through kinetic studies and spectra anylysis. (2) to establish much more sensitive methods for simultaneous separations and determinations of veterinary drug residues by use of the FI-UOTMC-CL and/or CE-UOTMC-CL; (3) to estimate performance index of these new detection methods and utilize these methods for deteminations of veterinary drug residues in food of animal origin.