干细胞是一类具有高度分化潜能的细胞，具有促进损伤组织再生并修复的特性。干细胞治疗是当今医学、动物医学、比较医学研究的热点。本项目组在前两个国自基金项目分别采用腹腔镜微创外科技术建立了小型猪肝脏缺血再灌注（I/R）及部分切除损伤标准模型(LLR)，并应用HRS和ADSCs分别对其进行干预，对比研究结果显示ADSCs对肝损伤较HRS具有更强保护及促进再生的作用。为了更深入探究ADSCs及其旁分泌作用的效果和作用机制，本实验应用小型猪ADSCs、ADSCs-CM和ADSCs-ES对小型猪腹腔镜微创肝损伤模型进行干预，研究不同干预方式对肝损伤后肝脏微环境和再生与修复的影响。进而客观评价ADSCs、ADSCs-CM和ADSCs-ES三种不同干预方式对肝损伤作用的差异性，筛选出最优的干预方式，并探索其保护作用机制。为动物医学和比较医学领域开展干细胞移植和无细胞治疗等科学研究提供新的技术手段和理论依据。

Stem cell are initial cells with high differentiation ability, which have the characteristics of promoting the regeneration and repair of damaged tissues. Stem cell is a hot pot currently in the domain of medicine, animal medicine and comparative medicine. In the first two projects of the National Natural Science Foundation of China, the standard models of liver ischemia-reperfusion (I/R) and Laparoscopic liver resection(LLR) in miniature pigs were established by laparoscopic minimally invasive surgery. HRS and ADSCs were used to intervene in the liver injury model. The results of the comparative study showed that ADSCs has stronger protection effect and promote regeneration ability than HRS in liver injury model. To further explore the effects and underlying mechanisms of ADSCs and their paracrine effects. This study will use ADSCs, ADSCs-CM and ADSCs-ES to intervene the liver injury model in miniature pigs. To study the effects of different intervention methods on liver microenvironment, regeneration and repair after liver injury. Then, objectively evaluate the difference of three different intervention methods of ADSCs, ADSCs-CM and ADSCs-ES on liver injury. The best intervention mode will be selected and its protective mechanism will be explored. This findings will provide a new technical means and theoretical basis for the research of stem cell transplantation and acellular therapy in the field of animal medicine and comparative medicine.