在比较医学方面，小型猪做为与人同源性高的实验动物和异种器官移植供体，现已被广泛的认可和应用。而对于小型猪肝脏损伤的干预和治疗是兽医学、比较医学等领域亟待解决的问题。本课题组在前期已经采用腹腔镜微创外科技术建立了小型猪肝脏缺血及部分切除损伤模型，该项目在以上研究的基础上，应用HRS和ADSCs分别对腹腔镜微创术肝损伤进行干预，探索HRS和ADSCs在肝脏缺血及部分切除损伤中的干预效果，同时将从分子水平和细胞水平对肝脏损伤微环境的变化进行探讨，从而深入研究不同因素干预对肝脏损伤微环境及修复能力的影响，为兽医学、比较医学领域开展器官移植、干细胞移植、干细胞治疗等科学研究提供重要的技术手段和实验动物模型。同时比较和评价HRS和ADSCs干预小型猪肝脏损伤修复的效果和影响因素，为人和动物肝脏损伤的修复以及合理治疗奠定基础。

Miniature pig, an ideal experimental animal and xenotranslantation donor, has been widely studied because of its similarity with human in comparative medicine.however, the inteventiontherapy of liver injury in miniature pig was urgent problem in the filed of veterinary and comparative medicine. Our group bulilded liver ischemia and injury resection model by laparoscopic minimally invasive surgical technique, then liver ischemia and injury resection were intervenely treated with saturated salt water and adipose-derived stem cell to explore its effect on the treatment of liver ischemia and injury resection and to monitor the change of the microenviroment of liver injury repair and.regeneration ability from the level of molecule and cell , which can provided significant experimental methods and theoretical basis for high-level scientific reseach such as organ transplantation ,stem cell transplantation,stem cell therapy in comparative and veterinary medicine filed. We also compared the saturated salt water and adipose-derived stem cell and model animal without inteventioning theorapy at the same condition and further evaluate the advantage of intervention theorapy ,this also lay the foundation for the treatment of liver injury.