番茄杂种优势明显，但番茄雄性不育材料在实际应用中局限较多，难以推广。加强对番茄雄性不育调控机制的研究，可望获得新的突破。随着基因组、转录组及蛋白组学的发展，从不同层次（DNA、RNA和蛋白质）上来探讨番茄花药发育调控的分子机制已成为可能。项目拟采用前期所克隆的番茄花药发育关键转录因子Aborted microspores(AMS)，分别构建超表达、干涉（RNAi）、基因敲除（CRISPR/Cas9）和绒毡层特异表达等5种表达载体，通过农杆菌介导法将载体转化番茄，获得人工创制的雄性不育突变体的同时，达到验证AMS基因功能的目的；于不同花药发育时期对转化番茄取样，进行转录组测序和蛋白组学定量，结合染色质免疫共沉淀（ChIP）和酵母双杂交技术，筛选其中差异表达和受AMS直接调控的下游基因，进一步解析AMS在整个番茄花药发育过程中的分子机制。项目可为创制番茄雄性不育研究奠定前期理论和应用材料基础。

Although hybrid superiority technique has made remarkable contribution to the promotion of many crops yield, in current hybrid breeding applications, the deficiency of male sterility resources and the instability of male sterility trait severely confined the F1 hybrid seed production. As the deeply researches and development of genomics, transcriptomics and proteomics, it is possible to explore the molecular mechanism of anther development. Based on the cloning and sequencing of the transcription factor AMS involving in anther development, our attempt is to construct the over-expression vector, RNAi vector, gene knock-out vector(CRIS/Cas9) and tapetum specific expression vector respectively, these vectors all contain AMS. Furthermore, we are planning to transform the vectors into tomato genome using Agrobacterium-mediated system. We are attempted to identify and select the transgenic male sterility plants which would be used to the test the AMS gene function. Our researches may reveal the molecular mechanism of AMS gene in the anther development of tomato after screening differential expression genes and the involved genes under the regulation of AMS factor, using RNA-seq, proteomics quantitative analysis and ChIP assay technique..　　This investigation would be of great potential importance in the field of the tomato hybrid breeding through transgene biotechnology. It may not only set the theoretic resource foundation in creating male sterility, but also has attractive application prospect to breed the new male sterility lines.