Characterization of the DREBA4-Type Transcription Factor (SlDREBA4), Which Contributes to Heat Tolerance in Tomatoes.
Characterization of the DREBA4-Type Transcription Factor (SlDREBA4), Which Contributes to Heat Tolerance in Tomatoes.
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有助于番茄耐热性的 DREBA4 型转录因子 (SlDREBA4) 的表征
DOI:
10.3389/fpls.2020.554520
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发表时间:
2020
影响因子:
5.6
通讯作者:
Zhao K
中科院分区:
文献类型:
--
作者:
Mao L;Deng M;Jiang S;Zhu H;Yang Z;Yue Y;Zhao K
Dehydration-responsive element binding (DREB) transcription factors play crucial regulatory roles in abiotic stress. The only DREB transcription factor in tomato (Solanum lycopersicum), SlDREBA4 (Accession No. MN197531), which was determined to be a DREBA4 subfamily member, was isolated from cv. Microtom using high-temperature-induced digital gene expression (DGE) profiling technology. The constitutive expression of SlDREBA4 was detected in different tissues of Microtom plants. In addition to responding to high temperature, SlDREBA4 was up-regulated after exposure to abscisic acid (ABA), cold, drought and high-salt conditions. Transgenic overexpression and silencing systems revealed that SlDREBA4 could alter the resistance of transgenic Microtom plants to heat stress by altering the content of osmolytes and stress hormones, and the activities of antioxidant enzymes at the physiologic level. Moreover, SlDREBA4 regulated the downstream gene expression of many heat shock proteins (Hsp), as well as calcium-binding protein enriched in the pathways of protein processing in endoplasmic reticulum (ko04141) and plant-pathogen interaction (ko04626) at the molecular level. SlDREBA4 also induces the expression of biosynthesis genes in jasmonic acid (JA), salicylic acid (SA), and ethylene (ETH), and specifically binds to the DRE elements (core sequence, A/GCCGAC) of the Hsp genes downstream from SlDREBA4. This study provides new genetic resources and rationales for tomato heat-tolerance breeding and the heat-related regulatory mechanisms of DREBs.
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影响因子:
5.6
作者:
Li S;Zhao Q;Zhu D;Yu J
通讯作者:
Yu J
影响因子:
5.6
作者:
Huang L;Wang Y;Wang W;Zhao X;Qin Q;Sun F;Hu F;Zhao Y;Li Z;Fu B;Li Z
通讯作者:
Li Z
影响因子:
2.4
作者:
Hu, Xiaoqing;Xu, Xuemei;Li, Chenghao
通讯作者:
Li, Chenghao
影响因子:
5.6
作者:
Byun MY;Cui LH;Lee J;Park H;Lee A;Kim WT;Lee H
通讯作者:
Lee H
影响因子:
2.9
作者:
Lovdal, Trond;Lillo, Cathrine
通讯作者:
Lillo, Cathrine