甲基-（5-羟甲基）呋喃-2-羧酸盐是玉米弯孢叶斑病菌产生的非寄主专化性毒素，是引起玉米叶斑病的重要致病因子。本项目在分子和生化水平上研究该毒素生物合成途径，对于揭示病菌产生毒素机理、筛选抗毒素作用靶标具有重要意义。通过比较基因组学、基因功能分析和生物化学等方法，筛选鉴定呋喃型毒素生物合成核心基因簇成员和毒素合成中间产物；在不同光照和营养条件下研究毒素生物合成核心基因簇成员互作性质、协同表达时序性及其与中间产物合成和积累的对应关系，构建呋喃型毒素生物合成核心基因簇成员相互关系连锁图；研究毒素生物合成核心基因簇编码酶蛋白活性中心结构特征和催化效应，综合提出玉米弯孢叶斑病菌呋喃型毒素生物合成分子和生化途径，填补国际空白，为全面揭示该病菌产生毒素分子机理奠定理论基础。

As an novel non-host specific toxin causing maize Curvularia leaf spot（Curvularia lunata）, methyl 5-(hydroxymethyl)furan-2-carboxylate is molecularly and biochemically investigated in its biosynthesis pathway. This study would make a great contribution to understanding the mechanism of the furotoid toxin production and favors screening gene targets from the pathogen potential for anti-toxin maize molecular assistant breeding. Using approaches of comparative genomics, molecular and biochemical analysis to clone the major members of core gene clusters related to toxin biosynthesis is performed mainly based on the homologous sequence identified in other genetic closely related pathogenic fungal species, then the function analysis with each of those cloned genes is carried out under varied conditions of illumination and nutrition. The interaction between members of the core cluster genes for synergistic expression, toxin subcellular accumulation process corresponding to the time course of gene dynamic expression are detected globally in order to construct a genetic linkage map for furanoid toxin biosynthesis. Furthermore the catalytic center of toxin synthase encoded by the gene clusters will be biochemically characterized. Taken together,a comprehensive biochemical and molecular pathway of furanoid toxin from Curvularia lunata in maize is proposed. The result as a first work in this field would build a solid basis for entirely understanding the toxin production mechanism.