木霉菌系统诱导玉米免疫反应是其生物防治玉米叶斑病重要途径。前期工作表明，哈茨木霉菌定殖玉米根系过程中分泌的外切葡聚糖酶以及释放的纤维二糖作为诱导抗性效应因子（激发子）激发玉米对叶斑病的免疫反应，但不清楚效应因子如何与根系发生互作、诱导宿主防御反应系统表达。本研究以大斑病菌、小斑病菌和弯孢叶斑病菌为靶标，鉴定哈茨木霉菌定殖玉米根系产生的外切葡聚糖酶和纤维二糖在根系皮层细胞上的受体结构及其亚细胞分布和代谢途径；研究效应因子与受体互作位点分子和生化特征，效应因子对受体构象的影响；示踪宿主防御反应信号（JA/ET、Ca2+、ROS等）从根系到叶片长距离转导通路；分析两种受体单一表达与协同表达时空规律及对宿主防御反应信号长距离转导的影响，揭示木霉菌-根系互作远程诱导玉米抗叶斑病分子机理。本研究将创新木霉菌诱导下的MAMPs/DAMPs协同作用的植物免疫学理论，为玉米微生态抗病分子育种奠定基础。

The systemic induced resistance to maize foliar disease by antagonistic Trichoderma is a major mechanism for biocontrol of maize foliar disease. Previous study showed that cellulase（exo-glucanase）and cellobiose as ISR effectors (elicitors) produced in the maize root colonization by T. harzianum is able to trigger maize leaf immunity response to foliar diseases, however, nowadays it is still unclear that what receptors in maize root cortex are responsible for the interaction with those T.harzianum effectors. To uncover the nature of both kinds of receptors and their way to trigger host plant defense response expression against infection of three kinds of foliar pathogen (Exserohilum turcicum, Bipolaris maydis, Curvularia lunata), we are going to identify the structure, subcellular distribution and degradation pathway of receptors (PRR) which is responsible for the recognition to exo-glucanase and cellubiose produced along the colonization of T.harzianum on root system. Furthermore the molecular interaction sites and biochemical characteristics between the interface of receptor-effectors as well as the changes of receptor conformation to fit binding effectors are investigaged. Meanwhile the signal long distance transduction pathway for triggering defense response is investigated from root to leaf. Then the differential expression modes of defense response initiated by the recognition of either single receptor or both receptors to effectors are characterized along time course in different maize organs. Taken together, a molecular mechanism is understood of the far distance-induced maize resistance against foliar diseases by the interaction of T.harzianum and host roots so as to innovate plant basic immunity theory supported by the synergistic action of MAMPs/DAMPs in the presence of antagonistic Trichoderma, and provide novel insight for performing molecular maize resistant breeding at micro-ecological level.