转录因子和miRNA在果蝇Toll通路免疫响应中的共调节作用研究

miRNA和转录因子(TF)在Toll通路中起重要调控作用，但TF和miRNA在果蝇Toll通路中的共调节作用却鲜有报道。我们发现TF和miRNA能共调节Toll通路中MyD88等基因，推测miRNA和TF共调节可能对Toll通路免疫响应起调控作用，为阐明果蝇Toll通路免疫响应中的TF和miRNA共调节作用及其机制，本项目拟对G+细菌刺激黑腹果蝇5个时间点的miRNA与mRNA转录组进行RNA-seq测序；构建G+细菌刺激下5个时间点Toll通路TF和miRNA共调节网络并与对照网络进行比较，发现TF-miRNA共调节模式的动态规律；对TF和miRNA共调节MyD88等基因进行功能研究，揭示TF和miRNA共调节果蝇Toll通路应对G+细菌感染响应的机制。项目完成对阐明TF和miRNA共调节在果蝇Toll通路免疫响应中的作用机制具有重要意义，并对动物TLR通路的深入研究提供重要的启示。

MicroRNAs(miRNAs) and Transcription factors (TFs) play important regulation roles in Toll signaling pathway of fruit fly. However, the co-regulatory roles mediated by TFs and miRNAs are poorly studied in Toll signaling pathway. Our previous works have shown that TF and miRNA might co-regulate MyD88 and orther genes in Toll signaling pathway of Drosophila melanogaster, implying that TFs and miRNAs could co-regulate the immune response of Toll signaling pathway. To reveal the co-regulatory roles and mechanisms mediated by TFs and miRNAs in Toll signaling pathway, in the current project, we will finish the RNA-seq sequencing of five different time points after G+bacteria stimulating Drosophila melanogaster male adult. Then, we will further construct the co-regulatory networks mediated by TFs and miRNAs in Toll signaling pathway of Drosophila melanogaster in five different time points after G+ bacterial stimulation, and compare the differences in regulation patterns of network within the five different time points, and discover the dynamic features of the co-regulatory networks mediated by TFs and miRNAs in Toll signaling pathway. Moreover, we will use functional genomics related methods to extensively study the functions of TF and miRNA co-regulation motifs with focusing on MyD88 to reveal the co-regulatory mechanisms mediated by TFs and miRNAs in Toll signaling pathway of Drosophila melanogaster during G+ bacterial infection. The completion of the project will provide valuable insights into the co-regulatory mechanisms mediated by TFs and miRNAs in fruit fly immune system, and also have important enlightenment to illuminate the molecular mechanism of the co-regulation mediated by TFs and miRNAs in Toll-like receptor immune signaling pathway of other insects and animals.