铁死亡是一种新发现的调控性细胞死亡过程，研究与铁死亡过程相关的生物活性物种的分析与检测对该过程相关疾病的预防和治疗具有指导意义。前期研究发现，荧光探针在检测铁死亡过程中的生物活性物种（如羟基自由基等）方面具有显著优势。然而，由于与铁死亡相关的生物活性物种繁多且其实时、原位检测的荧光探针仍非常缺乏，因此，需要进一步发展多种新的荧光探针以深入阐明不同物种与铁死亡过程之间的关系。对此，本申请项目拟设计合成新的有机小分子荧光探针，并在分子、细胞和活体三个层次上开展与铁死亡相关的生物活性物种（如羟基自由基、巯基物种、丙二醛）的原位、实时荧光成像分析，以揭示更多相关物种在铁死亡过程中的变化规律和作用机制，进而为铁死亡相关疾病的防治和药物研发提供新的参考。

Ferroptosis is a newly proposed regulated cell death process. Studies on bioactive species associated with this process may offer new inspiration for disease prevention and treatment. In our previous studies, fluorescence probes exhibited great superiority in the detection of bioactive species (such as hydroxyl radical) during ferroptosis. However, due to the variety of bioactive species in ferroptosis process, it’s urgently necessary to develop more efficient fluorescence probes for the in-situ fluorescence imaging of these bioactive species and illuminating their relationships with ferroptosis. Thus, the current proposal will focus on developing new fluorescence probes for bioactive species associated with ferroptosis (for instance hydroxyl radical, thiol species and malondialdehyde), revealing the changing behavior and molecular mechanism of these species in ferroptosis. It’s hoped that the selective and sensitive fluorescence imaging of these species in ferroptosis may afford new perspective for the treatment and drug design of the ferroptosis-associated diseases.